Expression of tryptophan 2,3-dioxygenase in mature granule cells of the adult mouse dentate gyrus

Koji Ohira, Hideo Hagihara, Keiko Toyama, Keizo Takao, Masaaki Kanai, Hiroshi Funakoshi, Toshikazu Nakamura, Tsuyoshi Miyakawa

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

New granule cells are continuously generated in the dentate gyrus of the adult hippocampus. During granule cell maturation, the mechanisms that differentiate new cells not only describe the degree of cell differentiation, but also crucially regulate the progression of cell differentiation. Here, we describe a gene, tryptophan 2,3-dioxygenase (TDO), whose expression distinguishes stem cells from more differentiated cells among the granule cells of the adult mouse dentate gyrus. The use of markers for proliferation, neural progenitors, and immature and mature granule cells indicated that TDO was expressed in mature cells and in some immature cells. In mice heterozygous for the alpha-isoform of calcium/calmodulin-dependent protein kinase II, in which dentate gyrus granule cells fail to mature normally, TDO immunoreactivity was substantially downregulated in the dentate gyrus granule cells. Moreover, a 5-bromo-2'-deoxyuridine labeling experiment revealed that new neurons began to express TDO between 2 and 4 wk after the neurons were generated, when the axons and dendrites of the granule cells developed and synaptogenesis occurred. These findings indicate that TDO might be required at a late-stage of granule cell development, such as during axonal and dendritic growth, synaptogenesis and its maturation.

Original languageEnglish
Article number26
JournalMolecular brain
Volume3
Issue number1
DOIs
Publication statusPublished - 16-09-2010

Fingerprint

Tryptophan Oxygenase
Dentate Gyrus
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Cell Differentiation
Neurons
Parahippocampal Gyrus
Calcium-Calmodulin-Dependent Protein Kinases
Bromodeoxyuridine
Dendrites
Axons

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Ohira, Koji ; Hagihara, Hideo ; Toyama, Keiko ; Takao, Keizo ; Kanai, Masaaki ; Funakoshi, Hiroshi ; Nakamura, Toshikazu ; Miyakawa, Tsuyoshi. / Expression of tryptophan 2,3-dioxygenase in mature granule cells of the adult mouse dentate gyrus. In: Molecular brain. 2010 ; Vol. 3, No. 1.
@article{a62867eabcfc41b6b6451bd0d9f1a2ff,
title = "Expression of tryptophan 2,3-dioxygenase in mature granule cells of the adult mouse dentate gyrus",
abstract = "New granule cells are continuously generated in the dentate gyrus of the adult hippocampus. During granule cell maturation, the mechanisms that differentiate new cells not only describe the degree of cell differentiation, but also crucially regulate the progression of cell differentiation. Here, we describe a gene, tryptophan 2,3-dioxygenase (TDO), whose expression distinguishes stem cells from more differentiated cells among the granule cells of the adult mouse dentate gyrus. The use of markers for proliferation, neural progenitors, and immature and mature granule cells indicated that TDO was expressed in mature cells and in some immature cells. In mice heterozygous for the alpha-isoform of calcium/calmodulin-dependent protein kinase II, in which dentate gyrus granule cells fail to mature normally, TDO immunoreactivity was substantially downregulated in the dentate gyrus granule cells. Moreover, a 5-bromo-2'-deoxyuridine labeling experiment revealed that new neurons began to express TDO between 2 and 4 wk after the neurons were generated, when the axons and dendrites of the granule cells developed and synaptogenesis occurred. These findings indicate that TDO might be required at a late-stage of granule cell development, such as during axonal and dendritic growth, synaptogenesis and its maturation.",
author = "Koji Ohira and Hideo Hagihara and Keiko Toyama and Keizo Takao and Masaaki Kanai and Hiroshi Funakoshi and Toshikazu Nakamura and Tsuyoshi Miyakawa",
year = "2010",
month = "9",
day = "16",
doi = "10.1186/1756-6606-3-26",
language = "English",
volume = "3",
journal = "Molecular Brain",
issn = "1756-6606",
publisher = "BioMed Central",
number = "1",

}

Expression of tryptophan 2,3-dioxygenase in mature granule cells of the adult mouse dentate gyrus. / Ohira, Koji; Hagihara, Hideo; Toyama, Keiko; Takao, Keizo; Kanai, Masaaki; Funakoshi, Hiroshi; Nakamura, Toshikazu; Miyakawa, Tsuyoshi.

In: Molecular brain, Vol. 3, No. 1, 26, 16.09.2010.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Expression of tryptophan 2,3-dioxygenase in mature granule cells of the adult mouse dentate gyrus

AU - Ohira, Koji

AU - Hagihara, Hideo

AU - Toyama, Keiko

AU - Takao, Keizo

AU - Kanai, Masaaki

AU - Funakoshi, Hiroshi

AU - Nakamura, Toshikazu

AU - Miyakawa, Tsuyoshi

PY - 2010/9/16

Y1 - 2010/9/16

N2 - New granule cells are continuously generated in the dentate gyrus of the adult hippocampus. During granule cell maturation, the mechanisms that differentiate new cells not only describe the degree of cell differentiation, but also crucially regulate the progression of cell differentiation. Here, we describe a gene, tryptophan 2,3-dioxygenase (TDO), whose expression distinguishes stem cells from more differentiated cells among the granule cells of the adult mouse dentate gyrus. The use of markers for proliferation, neural progenitors, and immature and mature granule cells indicated that TDO was expressed in mature cells and in some immature cells. In mice heterozygous for the alpha-isoform of calcium/calmodulin-dependent protein kinase II, in which dentate gyrus granule cells fail to mature normally, TDO immunoreactivity was substantially downregulated in the dentate gyrus granule cells. Moreover, a 5-bromo-2'-deoxyuridine labeling experiment revealed that new neurons began to express TDO between 2 and 4 wk after the neurons were generated, when the axons and dendrites of the granule cells developed and synaptogenesis occurred. These findings indicate that TDO might be required at a late-stage of granule cell development, such as during axonal and dendritic growth, synaptogenesis and its maturation.

AB - New granule cells are continuously generated in the dentate gyrus of the adult hippocampus. During granule cell maturation, the mechanisms that differentiate new cells not only describe the degree of cell differentiation, but also crucially regulate the progression of cell differentiation. Here, we describe a gene, tryptophan 2,3-dioxygenase (TDO), whose expression distinguishes stem cells from more differentiated cells among the granule cells of the adult mouse dentate gyrus. The use of markers for proliferation, neural progenitors, and immature and mature granule cells indicated that TDO was expressed in mature cells and in some immature cells. In mice heterozygous for the alpha-isoform of calcium/calmodulin-dependent protein kinase II, in which dentate gyrus granule cells fail to mature normally, TDO immunoreactivity was substantially downregulated in the dentate gyrus granule cells. Moreover, a 5-bromo-2'-deoxyuridine labeling experiment revealed that new neurons began to express TDO between 2 and 4 wk after the neurons were generated, when the axons and dendrites of the granule cells developed and synaptogenesis occurred. These findings indicate that TDO might be required at a late-stage of granule cell development, such as during axonal and dendritic growth, synaptogenesis and its maturation.

UR - http://www.scopus.com/inward/record.url?scp=77956463689&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956463689&partnerID=8YFLogxK

U2 - 10.1186/1756-6606-3-26

DO - 10.1186/1756-6606-3-26

M3 - Article

C2 - 20815922

AN - SCOPUS:77956463689

VL - 3

JO - Molecular Brain

JF - Molecular Brain

SN - 1756-6606

IS - 1

M1 - 26

ER -