Expressions of PDGF receptor alpha, c-Kit and Flk1 genes clustering in mouse chromosome 5 define distinct subsets of nascent mesodermal cells

Hiroshi Kataoka, Nobuyuki Takakura, Satomi Nishikawa, Kunihiro Tsuchida, Hiroaki Kodama, Takahiro Kunisada, Werner Risau, Toru Kita, Shin Lchi Nishikawa

Research output: Contribution to journalArticle

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Abstract

In gastrulating embryos, various types of cells are generated before differentiation into specific lineages. The mesoderm of the gastrulating mouse embryo represents a group of such intermediate cells. PDGF receptor alpha (PDGFRα), c-Kit and fetal liver kinase 1 (Flk1) are expressed in distinctive mesodermal derivatives of post-gastrulation embryos. Their expressions during gastrulation were examined by whole mount immunostaining with monoclonal antibodies against these three receptors. The antibodies stained different mesodermal subsets in gastrulating embryos. Flow cytometry of head fold stage embryos revealed that Flk1+ mesodermal cells could be further classified by the level of c-Kit expression. To examine the possibility that hematopoietic cell differentiation is initiated from the Flk1+ mesoderm, embryonic stem (ES) cells were cultured on the OP9 or PA6 stromal cell layer; the former but not the latter supported in vitro hematopoiesis from ES cells. Flk1+ cells were detected only on the OP9 cell layer from day 3 of differentiation before the appearance of hematopoietic cells. Thus, Flk1+ cells will be required for in vitro ES cell differentiation into hematopoietic cells. The results suggest that these three receptor tyrosine kinases will be useful for defining and sorting subsets of mesodermal cells from embryos or in vitro cultured ES cells.

Original languageEnglish
Pages (from-to)729-740
Number of pages12
JournalDevelopment Growth and Differentiation
Volume39
Issue number6
DOIs
Publication statusPublished - 01-01-1997

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Platelet-Derived Growth Factor alpha Receptor
Vascular Endothelial Growth Factor Receptor-2
Chromosomes, Human, Pair 5
Cluster Analysis
Embryonic Structures
Embryonic Stem Cells
Genes
Gastrulation
Mesoderm
Cell Differentiation
Hematopoiesis
Receptor Protein-Tyrosine Kinases
Stromal Cells
Flow Cytometry
Head
Monoclonal Antibodies

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

Cite this

Kataoka, Hiroshi ; Takakura, Nobuyuki ; Nishikawa, Satomi ; Tsuchida, Kunihiro ; Kodama, Hiroaki ; Kunisada, Takahiro ; Risau, Werner ; Kita, Toru ; Nishikawa, Shin Lchi. / Expressions of PDGF receptor alpha, c-Kit and Flk1 genes clustering in mouse chromosome 5 define distinct subsets of nascent mesodermal cells. In: Development Growth and Differentiation. 1997 ; Vol. 39, No. 6. pp. 729-740.
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Expressions of PDGF receptor alpha, c-Kit and Flk1 genes clustering in mouse chromosome 5 define distinct subsets of nascent mesodermal cells. / Kataoka, Hiroshi; Takakura, Nobuyuki; Nishikawa, Satomi; Tsuchida, Kunihiro; Kodama, Hiroaki; Kunisada, Takahiro; Risau, Werner; Kita, Toru; Nishikawa, Shin Lchi.

In: Development Growth and Differentiation, Vol. 39, No. 6, 01.01.1997, p. 729-740.

Research output: Contribution to journalArticle

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AU - Kataoka, Hiroshi

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AU - Risau, Werner

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AU - Nishikawa, Shin Lchi

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