Extended-spectrum AmpC cephalosporinase in Acinetobacter baumannii

ADC-56 confers resistance to cefepime

Guo Bao Tian, Jennifer M. Adams-Haduch, Magdalena Taracila, Robert A. Bonomo, Hong Ning Wang, Yohei Doi

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

ADC-56, a novel extended-spectrum AmpC (ESAC) β-lactamase, was identified in an Acinetobacter baumannii clinical isolate. ADC-56 possessed an R148Q change compared with its putative progenitor, ADC-30, which enabled it to hydrolyze cefepime. Molecular modeling suggested that R148 interacted with Q267, E272, and I291 through a hydrogen bond network which constrained the H-10 helix. This permitted cefepime to undergo conformational changes in the active site, with the carboxyl interacting with R340, likely allowing for better binding and turnover.

Original languageEnglish
Pages (from-to)4922-4925
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume55
Issue number10
DOIs
Publication statusPublished - 01-10-2011
Externally publishedYes

Fingerprint

Cephalosporinase
Acinetobacter baumannii
Hydrogen
Catalytic Domain
cefepime

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Tian, Guo Bao ; Adams-Haduch, Jennifer M. ; Taracila, Magdalena ; Bonomo, Robert A. ; Wang, Hong Ning ; Doi, Yohei. / Extended-spectrum AmpC cephalosporinase in Acinetobacter baumannii : ADC-56 confers resistance to cefepime. In: Antimicrobial Agents and Chemotherapy. 2011 ; Vol. 55, No. 10. pp. 4922-4925.
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Extended-spectrum AmpC cephalosporinase in Acinetobacter baumannii : ADC-56 confers resistance to cefepime. / Tian, Guo Bao; Adams-Haduch, Jennifer M.; Taracila, Magdalena; Bonomo, Robert A.; Wang, Hong Ning; Doi, Yohei.

In: Antimicrobial Agents and Chemotherapy, Vol. 55, No. 10, 01.10.2011, p. 4922-4925.

Research output: Contribution to journalArticle

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