Extended-spectrum AmpC cephalosporinase in Acinetobacter baumannii: ADC-56 confers resistance to cefepime

Guo Bao Tian; Jennifer M. Adams-Haduch; Magdalena Taracila; Robert A. Bonomo; Hong Ning Wang; Yohei Doi

doi:10.1128/AAC.00704-11

Extended-spectrum AmpC cephalosporinase in Acinetobacter baumannii: ADC-56 confers resistance to cefepime

Guo Bao Tian
, Jennifer M. Adams-Haduch
, Magdalena Taracila
, Robert A. Bonomo
, Hong Ning Wang
, Yohei Doi

Research output: Contribution to journal › Article › peer-review

63 Citations (Scopus)

Abstract

ADC-56, a novel extended-spectrum AmpC (ESAC) β-lactamase, was identified in an Acinetobacter baumannii clinical isolate. ADC-56 possessed an R148Q change compared with its putative progenitor, ADC-30, which enabled it to hydrolyze cefepime. Molecular modeling suggested that R148 interacted with Q267, E272, and I291 through a hydrogen bond network which constrained the H-10 helix. This permitted cefepime to undergo conformational changes in the active site, with the carboxyl interacting with R340, likely allowing for better binding and turnover.

Original language	English
Pages (from-to)	4922-4925
Number of pages	4
Journal	Antimicrobial agents and chemotherapy
Volume	55
Issue number	10
DOIs	https://doi.org/10.1128/AAC.00704-11
Publication status	Published - 10-2011
Externally published	Yes

All Science Journal Classification (ASJC) codes

Pharmacology
Pharmacology (medical)
Infectious Diseases

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10.1128/AAC.00704-11

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title = "Extended-spectrum AmpC cephalosporinase in Acinetobacter baumannii: ADC-56 confers resistance to cefepime",

abstract = "ADC-56, a novel extended-spectrum AmpC (ESAC) β-lactamase, was identified in an Acinetobacter baumannii clinical isolate. ADC-56 possessed an R148Q change compared with its putative progenitor, ADC-30, which enabled it to hydrolyze cefepime. Molecular modeling suggested that R148 interacted with Q267, E272, and I291 through a hydrogen bond network which constrained the H-10 helix. This permitted cefepime to undergo conformational changes in the active site, with the carboxyl interacting with R340, likely allowing for better binding and turnover.",

author = "Tian, \{Guo Bao\} and Adams-Haduch, \{Jennifer M.\} and Magdalena Taracila and Bonomo, \{Robert A.\} and Wang, \{Hong Ning\} and Yohei Doi",

year = "2011",

month = oct,

doi = "10.1128/AAC.00704-11",

language = "English",

volume = "55",

pages = "4922--4925",

journal = "Antimicrobial agents and chemotherapy",

issn = "0066-4804",

publisher = "American Society for Microbiology",

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T1 - Extended-spectrum AmpC cephalosporinase in Acinetobacter baumannii

T2 - ADC-56 confers resistance to cefepime

AU - Tian, Guo Bao

AU - Adams-Haduch, Jennifer M.

AU - Taracila, Magdalena

AU - Bonomo, Robert A.

AU - Wang, Hong Ning

AU - Doi, Yohei

PY - 2011/10

Y1 - 2011/10

N2 - ADC-56, a novel extended-spectrum AmpC (ESAC) β-lactamase, was identified in an Acinetobacter baumannii clinical isolate. ADC-56 possessed an R148Q change compared with its putative progenitor, ADC-30, which enabled it to hydrolyze cefepime. Molecular modeling suggested that R148 interacted with Q267, E272, and I291 through a hydrogen bond network which constrained the H-10 helix. This permitted cefepime to undergo conformational changes in the active site, with the carboxyl interacting with R340, likely allowing for better binding and turnover.

AB - ADC-56, a novel extended-spectrum AmpC (ESAC) β-lactamase, was identified in an Acinetobacter baumannii clinical isolate. ADC-56 possessed an R148Q change compared with its putative progenitor, ADC-30, which enabled it to hydrolyze cefepime. Molecular modeling suggested that R148 interacted with Q267, E272, and I291 through a hydrogen bond network which constrained the H-10 helix. This permitted cefepime to undergo conformational changes in the active site, with the carboxyl interacting with R340, likely allowing for better binding and turnover.

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UR - https://www.scopus.com/pages/publications/80052863427#tab=citedBy

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M3 - Article

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AN - SCOPUS:80052863427

SN - 0066-4804

VL - 55

SP - 4922

EP - 4925

JO - Antimicrobial agents and chemotherapy

JF - Antimicrobial agents and chemotherapy

IS - 10

ER -

Extended-spectrum AmpC cephalosporinase in Acinetobacter baumannii: ADC-56 confers resistance to cefepime

Abstract

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