Extracellular proteoglycan decorin maintains human hair follicle stem cells

Katsuma Miyachi, Takaaki Yamada, Mika Kawagishi-Hotta, Yuichi Hasebe, Yasushi Date, Seiji Hasegawa, Masaru Arima, Yohei Iwata, Tsukane Kobayashi, Shigeki Numata, Naoki Yamamoto, Satoru Nakata, Kazumitsu Sugiura, Hirohiko Akamatsu

Research output: Contribution to journalArticle

Abstract

Hair follicle stem cells (HFSC) are localized in the bulge region of the hair follicle and play a role in producing hair. Recently, it has been shown that the number of HFSC decreases with age, which is thought to be a cause of senile alopecia. Therefore, maintaining HFSC may be key for the prevention of age-related hair loss, but the regulatory mechanisms of HFSC and the effects of aging on them are largely unknown. In general, stem cells are known to require regulatory factors in the pericellular microenvironment, termed the stem cell niche, to maintain their cell function. In this study, we focused on the extracellular matrix proteoglycan decorin (DCN) as a candidate factor for maintaining the human HFSC niche. Gene expression analysis showed that DCN was highly expressed in the bulge region. We observed decreases in DCN expression as well as the number of KRT15-positive HFSC with age. In vitro experiments with human plucked hair-derived HFSC revealed that HFSC lost their undifferentiated state with increasing passages, and prior to this change a decrease in DCN expression was observed. Furthermore, knockdown of DCN promoted HFSC differentiation. In contrast, when HFSC were cultured on DCN-coated plates, they showed an even more undifferentiated state. From these results, as a novel mechanism for maintaining HFSC, it was suggested that DCN functions as a stem cell niche component, and that the deficit of HFSC maintenance caused by a reduction in DCN expression could be a cause of age-related hair loss.

Original languageEnglish
Pages (from-to)1403-1410
Number of pages8
JournalJournal of Dermatology
Volume45
Issue number12
DOIs
Publication statusPublished - 01-12-2018

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Decorin
Hair Follicle
Proteoglycans
Stem Cells
Stem Cell Niche
Alopecia
Hair
Cell Aging
Cellular Structures

All Science Journal Classification (ASJC) codes

  • Dermatology

Cite this

Miyachi, K., Yamada, T., Kawagishi-Hotta, M., Hasebe, Y., Date, Y., Hasegawa, S., ... Akamatsu, H. (2018). Extracellular proteoglycan decorin maintains human hair follicle stem cells. Journal of Dermatology, 45(12), 1403-1410. https://doi.org/10.1111/1346-8138.14678
Miyachi, Katsuma ; Yamada, Takaaki ; Kawagishi-Hotta, Mika ; Hasebe, Yuichi ; Date, Yasushi ; Hasegawa, Seiji ; Arima, Masaru ; Iwata, Yohei ; Kobayashi, Tsukane ; Numata, Shigeki ; Yamamoto, Naoki ; Nakata, Satoru ; Sugiura, Kazumitsu ; Akamatsu, Hirohiko. / Extracellular proteoglycan decorin maintains human hair follicle stem cells. In: Journal of Dermatology. 2018 ; Vol. 45, No. 12. pp. 1403-1410.
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abstract = "Hair follicle stem cells (HFSC) are localized in the bulge region of the hair follicle and play a role in producing hair. Recently, it has been shown that the number of HFSC decreases with age, which is thought to be a cause of senile alopecia. Therefore, maintaining HFSC may be key for the prevention of age-related hair loss, but the regulatory mechanisms of HFSC and the effects of aging on them are largely unknown. In general, stem cells are known to require regulatory factors in the pericellular microenvironment, termed the stem cell niche, to maintain their cell function. In this study, we focused on the extracellular matrix proteoglycan decorin (DCN) as a candidate factor for maintaining the human HFSC niche. Gene expression analysis showed that DCN was highly expressed in the bulge region. We observed decreases in DCN expression as well as the number of KRT15-positive HFSC with age. In vitro experiments with human plucked hair-derived HFSC revealed that HFSC lost their undifferentiated state with increasing passages, and prior to this change a decrease in DCN expression was observed. Furthermore, knockdown of DCN promoted HFSC differentiation. In contrast, when HFSC were cultured on DCN-coated plates, they showed an even more undifferentiated state. From these results, as a novel mechanism for maintaining HFSC, it was suggested that DCN functions as a stem cell niche component, and that the deficit of HFSC maintenance caused by a reduction in DCN expression could be a cause of age-related hair loss.",
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Miyachi, K, Yamada, T, Kawagishi-Hotta, M, Hasebe, Y, Date, Y, Hasegawa, S, Arima, M, Iwata, Y, Kobayashi, T, Numata, S, Yamamoto, N, Nakata, S, Sugiura, K & Akamatsu, H 2018, 'Extracellular proteoglycan decorin maintains human hair follicle stem cells', Journal of Dermatology, vol. 45, no. 12, pp. 1403-1410. https://doi.org/10.1111/1346-8138.14678

Extracellular proteoglycan decorin maintains human hair follicle stem cells. / Miyachi, Katsuma; Yamada, Takaaki; Kawagishi-Hotta, Mika; Hasebe, Yuichi; Date, Yasushi; Hasegawa, Seiji; Arima, Masaru; Iwata, Yohei; Kobayashi, Tsukane; Numata, Shigeki; Yamamoto, Naoki; Nakata, Satoru; Sugiura, Kazumitsu; Akamatsu, Hirohiko.

In: Journal of Dermatology, Vol. 45, No. 12, 01.12.2018, p. 1403-1410.

Research output: Contribution to journalArticle

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T1 - Extracellular proteoglycan decorin maintains human hair follicle stem cells

AU - Miyachi, Katsuma

AU - Yamada, Takaaki

AU - Kawagishi-Hotta, Mika

AU - Hasebe, Yuichi

AU - Date, Yasushi

AU - Hasegawa, Seiji

AU - Arima, Masaru

AU - Iwata, Yohei

AU - Kobayashi, Tsukane

AU - Numata, Shigeki

AU - Yamamoto, Naoki

AU - Nakata, Satoru

AU - Sugiura, Kazumitsu

AU - Akamatsu, Hirohiko

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Hair follicle stem cells (HFSC) are localized in the bulge region of the hair follicle and play a role in producing hair. Recently, it has been shown that the number of HFSC decreases with age, which is thought to be a cause of senile alopecia. Therefore, maintaining HFSC may be key for the prevention of age-related hair loss, but the regulatory mechanisms of HFSC and the effects of aging on them are largely unknown. In general, stem cells are known to require regulatory factors in the pericellular microenvironment, termed the stem cell niche, to maintain their cell function. In this study, we focused on the extracellular matrix proteoglycan decorin (DCN) as a candidate factor for maintaining the human HFSC niche. Gene expression analysis showed that DCN was highly expressed in the bulge region. We observed decreases in DCN expression as well as the number of KRT15-positive HFSC with age. In vitro experiments with human plucked hair-derived HFSC revealed that HFSC lost their undifferentiated state with increasing passages, and prior to this change a decrease in DCN expression was observed. Furthermore, knockdown of DCN promoted HFSC differentiation. In contrast, when HFSC were cultured on DCN-coated plates, they showed an even more undifferentiated state. From these results, as a novel mechanism for maintaining HFSC, it was suggested that DCN functions as a stem cell niche component, and that the deficit of HFSC maintenance caused by a reduction in DCN expression could be a cause of age-related hair loss.

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Miyachi K, Yamada T, Kawagishi-Hotta M, Hasebe Y, Date Y, Hasegawa S et al. Extracellular proteoglycan decorin maintains human hair follicle stem cells. Journal of Dermatology. 2018 Dec 1;45(12):1403-1410. https://doi.org/10.1111/1346-8138.14678