Faecal carriage of CTX-M-15-producing Klebsiella pneumoniae in patients with acute gastroenteritis

Muzaheed, Yohei Doi, J. M. Adams-Haduch, C. T. Shivannavar, David L. Paterson, S. M. Gaddad

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Background & objectives: Data on extended-spectrum β-lactamases (ESBLs) produced by Gram-negative bacteria including Klebsiella pneumoniae especially molecular types of ESBL genes from India are limited. The present study was conducted to investigate the carriage and ESBL contents of multidrug-resistant K. pneumoniae recovered from patients with gastroenteritis in a rural village in southern India. Methods: Nine K. pneumoniae isolates obtained from 45 stool samples from patients with gastroenteritis from one rural and two urban sites, in southern India were included in the study. Antibiotic susceptibility testing, PCR analysis and sequencing were conducted to characterize the ESBL genes. Clonal relatedness was assessed by pulsed-field gel electrophoresis (PFGE). Results: All the isolates were found to be resistant to at least one of the third generation cephalosporins tested. All the study isolates were confirmed to produce ESBLs. PCR and sequencing revealed the responsible gene to be blaCTX-M-15.blaTEM and blaSHV were absent. PFGE indicated that five of seven isolates from villagers were genetically closely related, and in turn were related to isolates from patients in two urban areas in this region. Interpretation & conclusions: Our findings showed that genetically-related isolates of K. pneumoniae producing CTX-M-15 were present in multiple areas in southern India. Larger studies need to be done in various geographical regions of the country to better define the molecular epidemiology of ESBL-producing K. pneumoniae and its clinical implications.

Original languageEnglish
Pages (from-to)599-602
Number of pages4
JournalIndian Journal of Medical Research
Volume129
Issue number5
Publication statusPublished - 21-09-2009
Externally publishedYes

Fingerprint

Gastroenteritis
Klebsiella pneumoniae
Genes
India
Electrophoresis
Gels
Pulsed Field Gel Electrophoresis
Geographical regions
Cephalosporins
Bacteria
Polymerase Chain Reaction
Molecular Epidemiology
Anti-Bacterial Agents
Gram-Negative Bacteria
Testing
galantide

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Muzaheed, Doi, Y., Adams-Haduch, J. M., Shivannavar, C. T., Paterson, D. L., & Gaddad, S. M. (2009). Faecal carriage of CTX-M-15-producing Klebsiella pneumoniae in patients with acute gastroenteritis. Indian Journal of Medical Research, 129(5), 599-602.
Muzaheed, ; Doi, Yohei ; Adams-Haduch, J. M. ; Shivannavar, C. T. ; Paterson, David L. ; Gaddad, S. M. / Faecal carriage of CTX-M-15-producing Klebsiella pneumoniae in patients with acute gastroenteritis. In: Indian Journal of Medical Research. 2009 ; Vol. 129, No. 5. pp. 599-602.
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Muzaheed, , Doi, Y, Adams-Haduch, JM, Shivannavar, CT, Paterson, DL & Gaddad, SM 2009, 'Faecal carriage of CTX-M-15-producing Klebsiella pneumoniae in patients with acute gastroenteritis', Indian Journal of Medical Research, vol. 129, no. 5, pp. 599-602.

Faecal carriage of CTX-M-15-producing Klebsiella pneumoniae in patients with acute gastroenteritis. / Muzaheed, ; Doi, Yohei; Adams-Haduch, J. M.; Shivannavar, C. T.; Paterson, David L.; Gaddad, S. M.

In: Indian Journal of Medical Research, Vol. 129, No. 5, 21.09.2009, p. 599-602.

Research output: Contribution to journalArticle

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Muzaheed , Doi Y, Adams-Haduch JM, Shivannavar CT, Paterson DL, Gaddad SM. Faecal carriage of CTX-M-15-producing Klebsiella pneumoniae in patients with acute gastroenteritis. Indian Journal of Medical Research. 2009 Sep 21;129(5):599-602.