FAK-mediated extracellular signals are essential for interkinetic nuclear migration and planar divisions in the neuroepithelium

Sachiko Tsuda, Tadao Kitagawa, Shigeo Takashima, Shuichi Asakawa, Nobuyoshi Shimizu, Hiroshi Mitani, Akihiro Shima, Makiko Tsutsumi, Hiroshi Hori, Kiyoshi Naruse, Yuji Ishikawa, Hiroyuki Takeda

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24 Citations (Scopus)

Abstract

During the development of the vertebrate nervous system, mitosis of neural progenitor cells takes place near the lumen, the apical side of the neural tube, through a characteristic movement of nuclei known as interkinetic nuclear migration (INM). Furthermore, during the proliferative period, neural progenitor cells exhibit planar cell divisions to produce equivalent daughter cells. Here, we examine the potential role of extracellular signals in INM and planar divisions using the medaka mutant tacobo (tab). This tab mutant shows pleiotropic phenotypes, including neurogenesis, and positional cloning identified tab as laminin γ1 (lamc1), providing a unique framework to study the role of extracelluar signals in neurogenesis. In tab mutant neural tubes, a number of nuclei exhibit abnormal patterns of migration leading to basally mislocalized mitosis. Furthermore, the orientation of cell division near the apical surface is randomized. Probably because of these defects, neurogenesis is accelerated in the tab neural tube. Detailed analyses demonstrate that extracellular signals mediated by the FAK pathway regulate INM and planar divisions in the neuroepithelium, possibly through interaction with the intracellular dynein-motor system.

Original languageEnglish
Pages (from-to)484-496
Number of pages13
JournalJournal of Cell Science
Volume123
Issue number3
DOIs
Publication statusPublished - 01-02-2010

All Science Journal Classification (ASJC) codes

  • Cell Biology

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    Tsuda, S., Kitagawa, T., Takashima, S., Asakawa, S., Shimizu, N., Mitani, H., Shima, A., Tsutsumi, M., Hori, H., Naruse, K., Ishikawa, Y., & Takeda, H. (2010). FAK-mediated extracellular signals are essential for interkinetic nuclear migration and planar divisions in the neuroepithelium. Journal of Cell Science, 123(3), 484-496. https://doi.org/10.1242/jcs.057851