Fibroblasts spread on immobilized fibrin monomer by mobilizing a β1- class integrin, together with a vitronectin receptor α(v)β3 on their surface

Shinji Asakura, Kazuki Niwa, Takako Tomozawa, Yong Ming Jin, Seiji Madoiwa, Yoichi Sakata, Takao Sakai, Hiroshi Funayama, Gilbu Soe, Fran Forgerty, Hajime Hirata, Michio Matsuda

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Human and murine fibroblasts were found to spread far more avidly on fibrin monomer monolayers than on immobilized fibrinogen, indicating that removal of fibrinopeptides by thrombin is a prerequisite for the fibrin- mediated augmentation of cell spreading. In fact, cell spreading was not efficiently augmented on monolayers of a thrombin-treated dysfibrinogen lacking the release of fibrinopeptide A due to an Aα Arg-16 → Cys substitution. Since a synthetic Arg-Gly-Asp (RGD)-containing peptide inhibited the fibrin-mediated cell spreading, subsequent dissociation of the carboxyl-terminal globular domain of the Aα-chains appears to render the RGD segments accessible to the cell-surface integrins. In support of this, fibrin-augmented cell spreading was inhibited by an antibody recognizing a 12-kDa peptide segment with γ Met-89 at its amino terminus, which is located in close association with the RGD segment at Aα 95-97 in the helical coiled- coil inter-domainal connector. The fibrin-mediated augmentation of cell spreading was inhibited not only by an antibody against human vitronectin receptor (LM 609) but also by an antibody against the β1 subunit of integrin (mAb13), suggesting that the β1-class integrin together with a vitronectin receptor, α(v)β3, is mobilized onto the surface of fibroblasts upon contact with the fibrin monomer monolayer.

Original languageEnglish
Pages (from-to)8824-8829
Number of pages6
JournalJournal of Biological Chemistry
Volume272
Issue number13
DOIs
Publication statusPublished - 28-03-1997
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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