TY - JOUR
T1 - Firefly luciferase as the reporter for transcriptional response to the environment in Escherichia coli
AU - Ryo, Masashi
AU - Oshikoshi, Yuta
AU - Doi, Shosei
AU - Motoki, Shogo
AU - Niimi, Atsuko
AU - Aoki, Setsuyuki
PY - 2013
Y1 - 2013
N2 - We demonstrate that firefly luciferase is a good reporter in Escherichia coli for transcription dynamics in response to the environment. E. coli strains, carrying a fusion of the promoter of the ycgZ gene and the coding region of the luciferase gene, showed transient bioluminescence on receiving blue light. This response was compromised in mutants lacking known regulators in manners consistent with each regulator's function. We also show that relA, a gene encoding a (p)ppGpp synthetase, affects ycgZ dynamics when nullified. Moreover, two unstable luciferase variants showed improved response dynamics and should be useful to study quick changes of gene expression.
AB - We demonstrate that firefly luciferase is a good reporter in Escherichia coli for transcription dynamics in response to the environment. E. coli strains, carrying a fusion of the promoter of the ycgZ gene and the coding region of the luciferase gene, showed transient bioluminescence on receiving blue light. This response was compromised in mutants lacking known regulators in manners consistent with each regulator's function. We also show that relA, a gene encoding a (p)ppGpp synthetase, affects ycgZ dynamics when nullified. Moreover, two unstable luciferase variants showed improved response dynamics and should be useful to study quick changes of gene expression.
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U2 - 10.1016/j.ab.2013.08.025
DO - 10.1016/j.ab.2013.08.025
M3 - Article
C2 - 24012794
AN - SCOPUS:84886081528
SN - 0003-2697
VL - 443
SP - 211
EP - 213
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -