First evidence for accumulation of protein-bound and protein-free pyrraline in human uremic plasma by mass spectrometry

Hiroko Odani; Toru Shinzato; Yoshihiro Matsumoto; Ichiro Takai; Shigeru Nakai; Masamiki Miwa; Norihisa Iwayama; Izumi Amano; Kenji Maeda

doi:10.1006/bbrc.1996.1013

First evidence for accumulation of protein-bound and protein-free pyrraline in human uremic plasma by mass spectrometry

Hiroko Odani, Toru Shinzato, Yoshihiro Matsumoto, Ichiro Takai, Shigeru Nakai, Masamiki Miwa, Norihisa Iwayama, Izumi Amano, Kenji Maeda

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

Glucose-derived advanced glycation end products (AGEs) cross-link proteins and cause various biological tissue damage. One of them, pyrraline [ε-2-(formyl-5-hydroxymethyl-pyrrol-1-yl) has been demonstrated by utilizing antibody to accumulate in plasma and sclerosed matrix of diabetic individuals, suggesting responsibility for diabetic complications. To elucidate the involvement of pyrraline in uremia, we examined the pyrraline levels in patients with chronic renal failure by a mass spectrometric approach. Here we show that protein-free pyrraline as well as pyrraline with binding protein are significantly increased in non-diabetic uremic plasma compared to healthy subjects. Our results suggest that circulating pyrraline could be a substance contributing to complications in uremia.

Original language	English
Pages (from-to)	237-241
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	224
Issue number	1
DOIs	https://doi.org/10.1006/bbrc.1996.1013
Publication status	Published - 05-07-1996
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1006/bbrc.1996.1013

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abstract = "Glucose-derived advanced glycation end products (AGEs) cross-link proteins and cause various biological tissue damage. One of them, pyrraline [ε-2-(formyl-5-hydroxymethyl-pyrrol-1-yl) has been demonstrated by utilizing antibody to accumulate in plasma and sclerosed matrix of diabetic individuals, suggesting responsibility for diabetic complications. To elucidate the involvement of pyrraline in uremia, we examined the pyrraline levels in patients with chronic renal failure by a mass spectrometric approach. Here we show that protein-free pyrraline as well as pyrraline with binding protein are significantly increased in non-diabetic uremic plasma compared to healthy subjects. Our results suggest that circulating pyrraline could be a substance contributing to complications in uremia.",

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AU - Odani, Hiroko

AU - Shinzato, Toru

AU - Matsumoto, Yoshihiro

AU - Takai, Ichiro

AU - Nakai, Shigeru

AU - Miwa, Masamiki

AU - Iwayama, Norihisa

AU - Amano, Izumi

AU - Maeda, Kenji

PY - 1996/7/5

Y1 - 1996/7/5

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First evidence for accumulation of protein-bound and protein-free pyrraline in human uremic plasma by mass spectrometry

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

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