Abstract
Twelve alternative methods to Draize eye irritation tests were preliminarily validated using 9 coded surfactants and physiological saline solution by 20 laboratories including a national institute, universities, kit suppliers, and cosmetics companies. The results indicated that intra- and interlaboratory variances were small as a whole except for the case of hemolysis by Tween 20; rank order of the cytotoxicity potentials of Triton X-100 among samples by cultured cell methods with serum differed greatly from those without serum; correlation coefficient between in vitro methods and in vivo Draize scores is higher than 0.8 in HET-CAM-trypan blue staining method, and cultured cell methods with serum and rank order correlation coefficients were higher than 0.9; correlation among cultured cell methods using serum was also high (r = 0.92-0.99).
Original language | English |
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Pages (from-to) | 89-94 |
Number of pages | 6 |
Journal | In Vitro Toxicology: Journal of Molecular and Cellular Toxicology |
Volume | 7 |
Issue number | 2 |
Publication status | Published - 1994 |
All Science Journal Classification (ASJC) codes
- Toxicology