Flow cytometric analysis of lymphocyte profiles in mediastinal lymphadenopathy of sarcoidosis

Ken Akao, Tomoyuki Minezawa, Naoki Yamamoto, Takuya Okamura, Takahiro Inoue, Kumiko Yamatsuta, Sakurako Uozu, Yasuhiro Goto, Masamichi Hayashi, Sumito Isogai, Masashi Kondo, Kazuyoshi Imaizumi

Research output: Contribution to journalArticle

Abstract

Lymphocyte profiles in mediastinal lymph nodes may reflect the immune status of patients with sarcoidosis. Endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) is useful for the diagnosis of diseases with mediastinal lymphadenopathy including sarcoidosis. The purpose of this study was to determine lymphocyte profiles of lymph nodes in sarcoidosis by analyzing EBUS-TBNA samples. We prepared single cell suspensions from EBUS-TBNA samples of mediastinal lymph nodes from patients with sarcoidosis or lung cancer and analyzed surface markers (CD3, CD4, CD8, CD19, CD25) and FoxP3 expression in the resultant lymphocytes using flow cytometry. We studied 26 patients with sarcoidosis and 16 with lung cancer with mediastinal lymph node metastases. In sarcoidosis, the CD4/CD8 ratio was significantly more elevated in lymph nodes than in bronchoalveolar lavage fluid (P<0.001), although both were strongly correlated. The CD4/CD8 ratio was significantly higher in stage I than in stage II both in the BAL fluid and lymph nodes. When compared with lung cancer lymph node metastasis, the CD4/CD8 ratio was significantly higher in sarcoidosis, whereas the CD3/CD19 ratio was significantly higher in lung cancer. The proportion of regulatory T cells (CD4+, CD25+, FoxP3 high) did not differ between sarcoidosis and lung cancer samples. Lymphocyte profiles in mediastinal lymphadenopathy can be analyzed by flow cytometry of EBUS-TBNA samples. These findings might help elucidate the immunopathology of sarcoidosis.

Original languageEnglish
Article numbere0206972
JournalPloS one
Volume13
Issue number11
DOIs
Publication statusPublished - 01-11-2018

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lymphatic diseases
Lymphocytes
Sarcoidosis
Needles
lymph nodes
lymphocytes
Ultrasonics
lung neoplasms
Lymph Nodes
Flow cytometry
Lung Neoplasms
CD4-CD8 Ratio
Dimercaprol
Fluids
T-cells
metastasis
flow cytometry
Suspensions
immunopathology
Flow Cytometry

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Akao, K., Minezawa, T., Yamamoto, N., Okamura, T., Inoue, T., Yamatsuta, K., ... Imaizumi, K. (2018). Flow cytometric analysis of lymphocyte profiles in mediastinal lymphadenopathy of sarcoidosis. PloS one, 13(11), [e0206972]. https://doi.org/10.1371/journal.pone.0206972
Akao, Ken ; Minezawa, Tomoyuki ; Yamamoto, Naoki ; Okamura, Takuya ; Inoue, Takahiro ; Yamatsuta, Kumiko ; Uozu, Sakurako ; Goto, Yasuhiro ; Hayashi, Masamichi ; Isogai, Sumito ; Kondo, Masashi ; Imaizumi, Kazuyoshi. / Flow cytometric analysis of lymphocyte profiles in mediastinal lymphadenopathy of sarcoidosis. In: PloS one. 2018 ; Vol. 13, No. 11.
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Akao, K, Minezawa, T, Yamamoto, N, Okamura, T, Inoue, T, Yamatsuta, K, Uozu, S, Goto, Y, Hayashi, M, Isogai, S, Kondo, M & Imaizumi, K 2018, 'Flow cytometric analysis of lymphocyte profiles in mediastinal lymphadenopathy of sarcoidosis', PloS one, vol. 13, no. 11, e0206972. https://doi.org/10.1371/journal.pone.0206972

Flow cytometric analysis of lymphocyte profiles in mediastinal lymphadenopathy of sarcoidosis. / Akao, Ken; Minezawa, Tomoyuki; Yamamoto, Naoki; Okamura, Takuya; Inoue, Takahiro; Yamatsuta, Kumiko; Uozu, Sakurako; Goto, Yasuhiro; Hayashi, Masamichi; Isogai, Sumito; Kondo, Masashi; Imaizumi, Kazuyoshi.

In: PloS one, Vol. 13, No. 11, e0206972, 01.11.2018.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Flow cytometric analysis of lymphocyte profiles in mediastinal lymphadenopathy of sarcoidosis

AU - Akao, Ken

AU - Minezawa, Tomoyuki

AU - Yamamoto, Naoki

AU - Okamura, Takuya

AU - Inoue, Takahiro

AU - Yamatsuta, Kumiko

AU - Uozu, Sakurako

AU - Goto, Yasuhiro

AU - Hayashi, Masamichi

AU - Isogai, Sumito

AU - Kondo, Masashi

AU - Imaizumi, Kazuyoshi

PY - 2018/11/1

Y1 - 2018/11/1

N2 - Lymphocyte profiles in mediastinal lymph nodes may reflect the immune status of patients with sarcoidosis. Endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) is useful for the diagnosis of diseases with mediastinal lymphadenopathy including sarcoidosis. The purpose of this study was to determine lymphocyte profiles of lymph nodes in sarcoidosis by analyzing EBUS-TBNA samples. We prepared single cell suspensions from EBUS-TBNA samples of mediastinal lymph nodes from patients with sarcoidosis or lung cancer and analyzed surface markers (CD3, CD4, CD8, CD19, CD25) and FoxP3 expression in the resultant lymphocytes using flow cytometry. We studied 26 patients with sarcoidosis and 16 with lung cancer with mediastinal lymph node metastases. In sarcoidosis, the CD4/CD8 ratio was significantly more elevated in lymph nodes than in bronchoalveolar lavage fluid (P<0.001), although both were strongly correlated. The CD4/CD8 ratio was significantly higher in stage I than in stage II both in the BAL fluid and lymph nodes. When compared with lung cancer lymph node metastasis, the CD4/CD8 ratio was significantly higher in sarcoidosis, whereas the CD3/CD19 ratio was significantly higher in lung cancer. The proportion of regulatory T cells (CD4+, CD25+, FoxP3 high) did not differ between sarcoidosis and lung cancer samples. Lymphocyte profiles in mediastinal lymphadenopathy can be analyzed by flow cytometry of EBUS-TBNA samples. These findings might help elucidate the immunopathology of sarcoidosis.

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