Fluorescence detection of intron lariat RNA with reduction-triggered fluorescent probes

Kazuhiro Furukawa, Hiroshi Abe, Yasutsugu Tamura, Rei Yoshimoto, Minoru Yoshida, Satoshi Tsuneda, Yoshihiro Ito

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Fluorescence through proximity: Reduction-triggered fluorescent probes bearing either a fluorescein derivative or triphenylphosphine bind sequence-specifically at the branch site of lariat RNA. The chemical reaction between the two substrates leads to the formation of unquenched fluorescein and the emission of a fluorescence signal (see picture). Prelariat and lariat structures can be distinguished by using this method.

Original languageEnglish
Pages (from-to)12020-12023
Number of pages4
JournalAngewandte Chemie - International Edition
Volume50
Issue number50
DOIs
Publication statusPublished - 09-12-2011

Fingerprint

Fluorescein
RNA
Fluorescent Dyes
Introns
Bearings (structural)
Fluorescence
Chemical reactions
Derivatives
Substrates
triphenylphosphine

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)

Cite this

Furukawa, Kazuhiro ; Abe, Hiroshi ; Tamura, Yasutsugu ; Yoshimoto, Rei ; Yoshida, Minoru ; Tsuneda, Satoshi ; Ito, Yoshihiro. / Fluorescence detection of intron lariat RNA with reduction-triggered fluorescent probes. In: Angewandte Chemie - International Edition. 2011 ; Vol. 50, No. 50. pp. 12020-12023.
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Fluorescence detection of intron lariat RNA with reduction-triggered fluorescent probes. / Furukawa, Kazuhiro; Abe, Hiroshi; Tamura, Yasutsugu; Yoshimoto, Rei; Yoshida, Minoru; Tsuneda, Satoshi; Ito, Yoshihiro.

In: Angewandte Chemie - International Edition, Vol. 50, No. 50, 09.12.2011, p. 12020-12023.

Research output: Contribution to journalArticle

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AU - Tsuneda, Satoshi

AU - Ito, Yoshihiro

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