Fluorescence detection of intron lariat RNA with reduction-triggered fluorescent probes

Kazuhiro Furukawa; Hiroshi Abe; Yasutsugu Tamura; Rei Yoshimoto; Minoru Yoshida; Satoshi Tsuneda; Yoshihiro Ito

doi:10.1002/anie.201104425

Fluorescence detection of intron lariat RNA with reduction-triggered fluorescent probes

Kazuhiro Furukawa, Hiroshi Abe, Yasutsugu Tamura, Rei Yoshimoto, Minoru Yoshida, Satoshi Tsuneda, Yoshihiro Ito

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Fluorescence through proximity: Reduction-triggered fluorescent probes bearing either a fluorescein derivative or triphenylphosphine bind sequence-specifically at the branch site of lariat RNA. The chemical reaction between the two substrates leads to the formation of unquenched fluorescein and the emission of a fluorescence signal (see picture). Prelariat and lariat structures can be distinguished by using this method.

Original language	English
Pages (from-to)	12020-12023
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	50
Issue number	50
DOIs	https://doi.org/10.1002/anie.201104425
Publication status	Published - 09-12-2011
Externally published	Yes

All Science Journal Classification (ASJC) codes

Catalysis
General Chemistry

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10.1002/anie.201104425

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abstract = "Fluorescence through proximity: Reduction-triggered fluorescent probes bearing either a fluorescein derivative or triphenylphosphine bind sequence-specifically at the branch site of lariat RNA. The chemical reaction between the two substrates leads to the formation of unquenched fluorescein and the emission of a fluorescence signal (see picture). Prelariat and lariat structures can be distinguished by using this method.",

author = "Kazuhiro Furukawa and Hiroshi Abe and Yasutsugu Tamura and Rei Yoshimoto and Minoru Yoshida and Satoshi Tsuneda and Yoshihiro Ito",

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AU - Furukawa, Kazuhiro

AU - Abe, Hiroshi

AU - Tamura, Yasutsugu

AU - Yoshimoto, Rei

AU - Yoshida, Minoru

AU - Tsuneda, Satoshi

AU - Ito, Yoshihiro

PY - 2011/12/9

Y1 - 2011/12/9

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Fluorescence detection of intron lariat RNA with reduction-triggered fluorescent probes

Abstract

All Science Journal Classification (ASJC) codes

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