Fluorescence detection of intron lariat RNA with reduction-triggered fluorescent probes

Kazuhiro Furukawa, Hiroshi Abe, Yasutsugu Tamura, Rei Yoshimoto, Minoru Yoshida, Satoshi Tsuneda, Yoshihiro Ito

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Fluorescence through proximity: Reduction-triggered fluorescent probes bearing either a fluorescein derivative or triphenylphosphine bind sequence-specifically at the branch site of lariat RNA. The chemical reaction between the two substrates leads to the formation of unquenched fluorescein and the emission of a fluorescence signal (see picture). Prelariat and lariat structures can be distinguished by using this method.

Original languageEnglish
Pages (from-to)12020-12023
Number of pages4
JournalAngewandte Chemie - International Edition
Volume50
Issue number50
DOIs
Publication statusPublished - 09-12-2011

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)

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  • Cite this

    Furukawa, K., Abe, H., Tamura, Y., Yoshimoto, R., Yoshida, M., Tsuneda, S., & Ito, Y. (2011). Fluorescence detection of intron lariat RNA with reduction-triggered fluorescent probes. Angewandte Chemie - International Edition, 50(50), 12020-12023. https://doi.org/10.1002/anie.201104425