Follistatin-related gene (FLRG) expression in human endometrium: Sex steroid hormones regulate the expression of FLRG in cultured human endometrial stromal cells

Hua Qin Wang, Koichi Takebayashi, Kunihiro Tsuchida, Masaki Nishimura, Yoichi Noda

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Follistatin-related gene (FLRG) encodes a novel secreted glycoprotein that is highly homologous to follistatin and binds activins and bone morphogenetic proteins, members of the TGFβ superfamily of growth/differentiation factors. FLRG protein inhibits activin-induced and bone morphogenetic protein-2-induced transcriptional responses in a dose-dependent manner, and its mRNA is abundantly expressed in human placenta, heart, aorta, testis, and adrenal gland. In this study we showed that FLRG mRNA was expressed in human endometrium across the menstrual cycle and in decidua of early pregnancy. In the proliferative phase of the menstrual cycle, FLRG protein was detected predominantly in the cytoplasm of endometrial epithelium. In the secretory phase and in early pregnancy, it was also detected in the nuclei of endometrial stromal cells. Using in vitro decidualization model, we demonstrated that 17β-estradiol plus progesterone, but not 17β-estradiol or progesterone alone, induced FLRG expression significantly. These results suggest that FLRG expression in endometrial stromal cells is regulated by the concerted action of ovarian steroid hormones via decidualization, and FLRG protein may participate in the regulation of stromal cell decidualization as a binding protein for members of TGFβ superfamily.

Original languageEnglish
Pages (from-to)4432-4439
Number of pages8
JournalJournal of Clinical Endocrinology and Metabolism
Volume88
Issue number9
DOIs
Publication statusPublished - 01-09-2003

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Follistatin
Follistatin-Related Proteins
Gonadal Steroid Hormones
Stromal Cells
Endometrium
Gene expression
Genes
Gene Expression
Activins
Progesterone
Estradiol
Growth Differentiation Factors
Decidua
Bone Morphogenetic Protein 2
Pregnancy
Messenger RNA
Bone Morphogenetic Proteins
Proteins
Follicular Phase
Menstrual Cycle

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

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Follistatin-related gene (FLRG) expression in human endometrium : Sex steroid hormones regulate the expression of FLRG in cultured human endometrial stromal cells. / Wang, Hua Qin; Takebayashi, Koichi; Tsuchida, Kunihiro; Nishimura, Masaki; Noda, Yoichi.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 88, No. 9, 01.09.2003, p. 4432-4439.

Research output: Contribution to journalArticle

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