Formation of actin stress fibers and focal adhesions enhanced by Rho- kinase

Mutsuki Amano, Kazuyasu Chihara, Kazushi Kimura, Yuko Fukata, Nao Nakamura, Yoshiharu Matsuura, Kozo Kaibuchi

Research output: Contribution to journalArticlepeer-review

918 Citations (Scopus)

Abstract

The small guanosine triphosphatase (GTPase) Rho is implicated in the formation of stress fibers and focal adhesions in fibroblasts stimulated by extracellular signals such as lysophosphatidic acid (LPA). Rho-kinase is activated by Rho and may mediate some biological effects of Rho. Microinjection of the catalytic domain of Rho-kinase into serum-starved Swiss 3T3 cells induced the formation of stress fibers and fOCal adhesions, whereas microinjection of the inactive catalytic domain, the Rho-binding domain, or the pleckstrin-homology domain inhibited the LPA-induced formation of stress fibers and focal adhesions. Thus, Rho-kinase appears to mediate signals from Rho and to induce the formation of stress fibers and focal adhesions.

Original languageEnglish
Pages (from-to)1308-1311
Number of pages4
JournalScience
Volume275
Issue number5304
DOIs
Publication statusPublished - 28-02-1997
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General

Fingerprint Dive into the research topics of 'Formation of actin stress fibers and focal adhesions enhanced by Rho- kinase'. Together they form a unique fingerprint.

Cite this