Frequent altered expression of fragile histidine triad protein in human colorectal adenomas

Hiroshi Morikawa, Yoshihito Nakagawa, Keisuke Hashimoto, Masami Niki, Yutaro Egashira, Ichiro Hirata, Kenichi Katsu, Yukihiro Akao

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Fragile histidine triad (FHIT) gene is involved in deletions on the short arm of chromosome 3 in various human cancers. We found that 47% of colorectal adenomas, which is a higher frequency than that of K-ras, showed altered expression of the Fhit protein by Western blot analysis. The amount of Fhit protein was inversely correlated with the degree of dysplasia. Importantly, 27% of low-grade dysplastic adenomas showed altered expression of Fhit protein. Additionally, expression of human Fhit protein in human colon carcinoma cell line SW480 exhibited a marked inhibition of growth and rendered SW480 cells highly susceptible to undergo apoptosis compared with control cells. These findings suggest that altered expression of the FHIT gene is a quite early aberration in the development of colorectal tumors and that Fhit protein may act as a tumor suppressor. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)205-210
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume278
Issue number1
DOIs
Publication statusPublished - 11-11-2000

Fingerprint

Adenoma
Proteins
Histidine
Tumors
Genes
Chromosomes, Human, Pair 3
Chromosomes
Aberrations
Colorectal Neoplasms
Neoplasms
Colon
Western Blotting
Cells
fragile histidine triad protein
Apoptosis
Carcinoma
Cell Line
Growth

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Morikawa, Hiroshi ; Nakagawa, Yoshihito ; Hashimoto, Keisuke ; Niki, Masami ; Egashira, Yutaro ; Hirata, Ichiro ; Katsu, Kenichi ; Akao, Yukihiro. / Frequent altered expression of fragile histidine triad protein in human colorectal adenomas. In: Biochemical and Biophysical Research Communications. 2000 ; Vol. 278, No. 1. pp. 205-210.
@article{945e0eb80d7b4ff5b39d6e301de1557e,
title = "Frequent altered expression of fragile histidine triad protein in human colorectal adenomas",
abstract = "Fragile histidine triad (FHIT) gene is involved in deletions on the short arm of chromosome 3 in various human cancers. We found that 47{\%} of colorectal adenomas, which is a higher frequency than that of K-ras, showed altered expression of the Fhit protein by Western blot analysis. The amount of Fhit protein was inversely correlated with the degree of dysplasia. Importantly, 27{\%} of low-grade dysplastic adenomas showed altered expression of Fhit protein. Additionally, expression of human Fhit protein in human colon carcinoma cell line SW480 exhibited a marked inhibition of growth and rendered SW480 cells highly susceptible to undergo apoptosis compared with control cells. These findings suggest that altered expression of the FHIT gene is a quite early aberration in the development of colorectal tumors and that Fhit protein may act as a tumor suppressor. (C) 2000 Academic Press.",
author = "Hiroshi Morikawa and Yoshihito Nakagawa and Keisuke Hashimoto and Masami Niki and Yutaro Egashira and Ichiro Hirata and Kenichi Katsu and Yukihiro Akao",
year = "2000",
month = "11",
day = "11",
doi = "10.1006/bbrc.2000.3771",
language = "English",
volume = "278",
pages = "205--210",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

Frequent altered expression of fragile histidine triad protein in human colorectal adenomas. / Morikawa, Hiroshi; Nakagawa, Yoshihito; Hashimoto, Keisuke; Niki, Masami; Egashira, Yutaro; Hirata, Ichiro; Katsu, Kenichi; Akao, Yukihiro.

In: Biochemical and Biophysical Research Communications, Vol. 278, No. 1, 11.11.2000, p. 205-210.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Frequent altered expression of fragile histidine triad protein in human colorectal adenomas

AU - Morikawa, Hiroshi

AU - Nakagawa, Yoshihito

AU - Hashimoto, Keisuke

AU - Niki, Masami

AU - Egashira, Yutaro

AU - Hirata, Ichiro

AU - Katsu, Kenichi

AU - Akao, Yukihiro

PY - 2000/11/11

Y1 - 2000/11/11

N2 - Fragile histidine triad (FHIT) gene is involved in deletions on the short arm of chromosome 3 in various human cancers. We found that 47% of colorectal adenomas, which is a higher frequency than that of K-ras, showed altered expression of the Fhit protein by Western blot analysis. The amount of Fhit protein was inversely correlated with the degree of dysplasia. Importantly, 27% of low-grade dysplastic adenomas showed altered expression of Fhit protein. Additionally, expression of human Fhit protein in human colon carcinoma cell line SW480 exhibited a marked inhibition of growth and rendered SW480 cells highly susceptible to undergo apoptosis compared with control cells. These findings suggest that altered expression of the FHIT gene is a quite early aberration in the development of colorectal tumors and that Fhit protein may act as a tumor suppressor. (C) 2000 Academic Press.

AB - Fragile histidine triad (FHIT) gene is involved in deletions on the short arm of chromosome 3 in various human cancers. We found that 47% of colorectal adenomas, which is a higher frequency than that of K-ras, showed altered expression of the Fhit protein by Western blot analysis. The amount of Fhit protein was inversely correlated with the degree of dysplasia. Importantly, 27% of low-grade dysplastic adenomas showed altered expression of Fhit protein. Additionally, expression of human Fhit protein in human colon carcinoma cell line SW480 exhibited a marked inhibition of growth and rendered SW480 cells highly susceptible to undergo apoptosis compared with control cells. These findings suggest that altered expression of the FHIT gene is a quite early aberration in the development of colorectal tumors and that Fhit protein may act as a tumor suppressor. (C) 2000 Academic Press.

UR - http://www.scopus.com/inward/record.url?scp=0034638604&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034638604&partnerID=8YFLogxK

U2 - 10.1006/bbrc.2000.3771

DO - 10.1006/bbrc.2000.3771

M3 - Article

VL - 278

SP - 205

EP - 210

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -