TY - JOUR
T1 - Frequent altered expression of fragile histidine triad protein in human colorectal adenomas
AU - Morikawa, Hiroshi
AU - Nakagawa, Yoshihito
AU - Hashimoto, Keisuke
AU - Niki, Masami
AU - Egashira, Yutaro
AU - Hirata, Ichiro
AU - Katsu, Kenichi
AU - Akao, Yukihiro
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid for Scientific Research by the Ministry of Education, Science, Sports, and Culture of Japan.
PY - 2000/11/11
Y1 - 2000/11/11
N2 - Fragile histidine triad (FHIT) gene is involved in deletions on the short arm of chromosome 3 in various human cancers. We found that 47% of colorectal adenomas, which is a higher frequency than that of K-ras, showed altered expression of the Fhit protein by Western blot analysis. The amount of Fhit protein was inversely correlated with the degree of dysplasia. Importantly, 27% of low-grade dysplastic adenomas showed altered expression of Fhit protein. Additionally, expression of human Fhit protein in human colon carcinoma cell line SW480 exhibited a marked inhibition of growth and rendered SW480 cells highly susceptible to undergo apoptosis compared with control cells. These findings suggest that altered expression of the FHIT gene is a quite early aberration in the development of colorectal tumors and that Fhit protein may act as a tumor suppressor. (C) 2000 Academic Press.
AB - Fragile histidine triad (FHIT) gene is involved in deletions on the short arm of chromosome 3 in various human cancers. We found that 47% of colorectal adenomas, which is a higher frequency than that of K-ras, showed altered expression of the Fhit protein by Western blot analysis. The amount of Fhit protein was inversely correlated with the degree of dysplasia. Importantly, 27% of low-grade dysplastic adenomas showed altered expression of Fhit protein. Additionally, expression of human Fhit protein in human colon carcinoma cell line SW480 exhibited a marked inhibition of growth and rendered SW480 cells highly susceptible to undergo apoptosis compared with control cells. These findings suggest that altered expression of the FHIT gene is a quite early aberration in the development of colorectal tumors and that Fhit protein may act as a tumor suppressor. (C) 2000 Academic Press.
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U2 - 10.1006/bbrc.2000.3771
DO - 10.1006/bbrc.2000.3771
M3 - Article
C2 - 11071873
AN - SCOPUS:0034638604
SN - 0006-291X
VL - 278
SP - 205
EP - 210
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -
