Frequent detection of human papilloma viruses in cervical dysplasia by PCR single-strand DNA-conformational polymorphism analysis

Hiroyuki Nakagawa, Kokichi Sugano, Takuma Fujii, Kaneyuki Kubushiro, Katsumi Tsukazaki, Shiro Nozawa

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background: To clarify the pathogenicity of multiple human papilloma virus (HPV) infection, we applied SSCP (single-strand DNA conformation polymorphism) analysis for cervical neoplastic lesions. Materials and Methods: Two hundred and sixty-six cervical swab specimens from normal cervix (n=64), cervical dysplasia (n=95), carcinoma in situ (n=79) and cervical cancer (n=28), were studied by nested PCR-SSCP analysis using L1 consensus primers. Results: In 95 samples of cervical dysplasia, HPV infection was detected in 98.9% (94 out of 95), multiple HPV infection was detected in 38.3% (36 out of 94). In 19 squamous cell carcinomas (SCC) and 9 adenocarcinomas, the detection rate of HPV infection was 84.2% (16 out of 19) and 55.6% (5 out of 9), respectively, and all HPV-positive cases showed infection of a single HPV, among which HPV 16 occupied 68.6% (11 out of 16) in SCC and HPV 18 occupied 100% (5 out of 5) in adenocarcinoma. Conclusion: Multiple HPV infections may be concerned with pathogenicity in cervical dysplasia; however, the single infection with only a few HPV types, such as type 16 in SCC and type 18 in adenocarcinoma, may play a role in cervical carcinogenesis.

Original languageEnglish
Pages (from-to)1655-1660
Number of pages6
JournalAnticancer Research
Volume22
Issue number3
Publication statusPublished - 14-08-2002
Externally publishedYes

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Papillomaviridae
Uterine Cervical Dysplasia
Polymerase Chain Reaction
DNA
Virus Diseases
Nucleic Acid Conformation
Squamous Cell Carcinoma
Adenocarcinoma
Virulence
Carcinoma in Situ
Infection

All Science Journal Classification (ASJC) codes

  • Cancer Research
  • Oncology

Cite this

Nakagawa, Hiroyuki ; Sugano, Kokichi ; Fujii, Takuma ; Kubushiro, Kaneyuki ; Tsukazaki, Katsumi ; Nozawa, Shiro. / Frequent detection of human papilloma viruses in cervical dysplasia by PCR single-strand DNA-conformational polymorphism analysis. In: Anticancer Research. 2002 ; Vol. 22, No. 3. pp. 1655-1660.
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abstract = "Background: To clarify the pathogenicity of multiple human papilloma virus (HPV) infection, we applied SSCP (single-strand DNA conformation polymorphism) analysis for cervical neoplastic lesions. Materials and Methods: Two hundred and sixty-six cervical swab specimens from normal cervix (n=64), cervical dysplasia (n=95), carcinoma in situ (n=79) and cervical cancer (n=28), were studied by nested PCR-SSCP analysis using L1 consensus primers. Results: In 95 samples of cervical dysplasia, HPV infection was detected in 98.9{\%} (94 out of 95), multiple HPV infection was detected in 38.3{\%} (36 out of 94). In 19 squamous cell carcinomas (SCC) and 9 adenocarcinomas, the detection rate of HPV infection was 84.2{\%} (16 out of 19) and 55.6{\%} (5 out of 9), respectively, and all HPV-positive cases showed infection of a single HPV, among which HPV 16 occupied 68.6{\%} (11 out of 16) in SCC and HPV 18 occupied 100{\%} (5 out of 5) in adenocarcinoma. Conclusion: Multiple HPV infections may be concerned with pathogenicity in cervical dysplasia; however, the single infection with only a few HPV types, such as type 16 in SCC and type 18 in adenocarcinoma, may play a role in cervical carcinogenesis.",
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Frequent detection of human papilloma viruses in cervical dysplasia by PCR single-strand DNA-conformational polymorphism analysis. / Nakagawa, Hiroyuki; Sugano, Kokichi; Fujii, Takuma; Kubushiro, Kaneyuki; Tsukazaki, Katsumi; Nozawa, Shiro.

In: Anticancer Research, Vol. 22, No. 3, 14.08.2002, p. 1655-1660.

Research output: Contribution to journalArticle

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