Frequent overexpression of CADM1/IGSF4 in lung adenocarcinoma

Yuka Kitamura, Gene Kurosawa, Miho Tanaka, Mariko Sumitomo, Chiho Muramatsu, Keiko Eguchi, Yasushi Akahori, Yoshitaka Iba, Hiroyuki Tsuda, Mototaka Sugiura, Yoshinobu Hattori, Yoshikazu Kurosawa

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14 Citations (Scopus)

Abstract

We reported comprehensive screening for antigens (Ags) overexpressed on various carcinomas via isolation of human monoclonal antibodies (mAbs) that may be therapeutic in a previous paper (Proc. Natl. Acad. Sci. USA 105, 7287-7292, 2008). Twenty-one distinct Ags highly expressed on several carcinomas were identified and 356 mAbs with unique sequences turned out to bind to one of the 21 Ags. Among them CADM1/IGSF4 which had been originally referred to as tumor suppressor lung cancer 1 (TSLC1) was included. Therefore we examined the expression of CADM1 in lung cancers in this study. Eight different anti CADM1 mAbs were used for immunohistochemical analysis of 29 fresh lung cancer specimens. Staining patterns were categorized to six groups based on the extent of positive staining and the localization of stained portions. While overexpression of CADM1 was observed on the cell surface of adenocarcinomas at a high frequency, around 60%, positive stainings were rarely observed on that of other lung carcinomas including squamous cell carcinomas. Moreover, some clones among the eight mAbs gave different staining patterns from those by the other clones against the same fresh specimen, suggesting presence of variant forms of CADM1 differentiated by mAbs.

Original languageEnglish
Pages (from-to)480-484
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume383
Issue number4
DOIs
Publication statusPublished - 12-06-2009

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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