TY - JOUR
T1 - Full genome-based characterization of G4P[6] rotavirus strains from diarrheic patients in Thailand
T2 - Evidence for independent porcine-to-human interspecies transmission events
AU - Tacharoenmuang, Ratana
AU - Guntapong, Ratigorn
AU - Upachai, Sompong
AU - Singchai, Phakapun
AU - Fukuda, Saori
AU - Ide, Tomihiko
AU - Hatazawa, Riona
AU - Sutthiwarakom, Karun
AU - Kongjorn, Santip
AU - Onvimala, Napa
AU - Luechakham, Tipsuda
AU - Ruchusatsawast, Kriangsak
AU - Kawamura, Yoshiki
AU - Sriwanthana, Busarawan
AU - Motomura, Kazushi
AU - Tatsumi, Masashi
AU - Takeda, Naokazu
AU - Yoshikawa, Tetsushi
AU - Murata, Takayuki
AU - Uppapong, Ballang
AU - Taniguchi, Koki
AU - Komoto, Satoshi
N1 - Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2021/8
Y1 - 2021/8
N2 - The exact evolutionary patterns of human G4P[6] rotavirus strains remain to be elucidated. Such strains possess unique and strain-specific genotype constellations, raising the question of whether G4P[6] strains are primarily transmitted via independent interspecies transmission or human-to-human transmission after interspecies transmission. Two G4P[6] rotavirus strains were identified in fecal specimens from hospitalized patients with severe diarrhea in Thailand, namely, DU2014-259 (RVA/Human-wt/THA/DU2014-259/2014/G4P[6]) and PK2015-1-0001 (RVA/Human-wt/THA/PK2015-1-0001/2015/G4P[6]). Here, we analyzed the full genomes of the two human G4P[6] strains, which provided the opportunity to study and confirm their evolutionary origin. On whole genome analysis, both strains exhibited a unique Wa-like genotype constellation of G4-P[6]-I1-R1-C1-M1-A8-N1-T1-E1-H1. The NSP1 genotype A8 is commonly found in porcine rotavirus strains. Furthermore, on phylogenetic analysis, each of the 11 genes of strains DU2014-259 and PK2015-1-0001 appeared to be of porcine origin. On the other hand, the two study strains consistently formed distinct clusters for nine of the 11 gene segments (VP4, VP6, VP1-VP3, and NSP2-NSP5), strongly indicating the occurrence of independent porcine-to-human interspecies transmission events. Our observations provide important insights into the origin of zoonotic G4P[6] strains, and into the dynamic interaction between porcine and human rotavirus strains.
AB - The exact evolutionary patterns of human G4P[6] rotavirus strains remain to be elucidated. Such strains possess unique and strain-specific genotype constellations, raising the question of whether G4P[6] strains are primarily transmitted via independent interspecies transmission or human-to-human transmission after interspecies transmission. Two G4P[6] rotavirus strains were identified in fecal specimens from hospitalized patients with severe diarrhea in Thailand, namely, DU2014-259 (RVA/Human-wt/THA/DU2014-259/2014/G4P[6]) and PK2015-1-0001 (RVA/Human-wt/THA/PK2015-1-0001/2015/G4P[6]). Here, we analyzed the full genomes of the two human G4P[6] strains, which provided the opportunity to study and confirm their evolutionary origin. On whole genome analysis, both strains exhibited a unique Wa-like genotype constellation of G4-P[6]-I1-R1-C1-M1-A8-N1-T1-E1-H1. The NSP1 genotype A8 is commonly found in porcine rotavirus strains. Furthermore, on phylogenetic analysis, each of the 11 genes of strains DU2014-259 and PK2015-1-0001 appeared to be of porcine origin. On the other hand, the two study strains consistently formed distinct clusters for nine of the 11 gene segments (VP4, VP6, VP1-VP3, and NSP2-NSP5), strongly indicating the occurrence of independent porcine-to-human interspecies transmission events. Our observations provide important insights into the origin of zoonotic G4P[6] strains, and into the dynamic interaction between porcine and human rotavirus strains.
UR - https://www.scopus.com/pages/publications/85107499067
UR - https://www.scopus.com/inward/citedby.url?scp=85107499067&partnerID=8YFLogxK
U2 - 10.1007/s11262-021-01851-y
DO - 10.1007/s11262-021-01851-y
M3 - Article
C2 - 34106412
AN - SCOPUS:85107499067
SN - 0920-8569
VL - 57
SP - 338
EP - 357
JO - Virus Genes
JF - Virus Genes
IS - 4
ER -
