β1-null GD25 fibroblasts adherent to vitronectin fail to bind the N- terminal 70-kDa matrix assembly domain of fibronectin or to assemble fibronectin (Sakai, T., Zhang, Q., Fassler, R., and Mosher, D. F. (1998) J. Cell Biol. 141, 527-538). We have made four observations that extend this finding. First, the presence of vitronectin on a substrate that otherwise can support fibronectin assembly has a dominant-negative effect on assembly. Second, the dominant-negative effect is lost when active β1A is expressed. Third, β1A containing the extracellular D130A inactivating mutation has a dominant-negative effect on fibronectin assembly. Fourth, β1-null cells adherent to vitronectin are fiat and lack filopodia, whereas β1-null cells adherent to fibronectin or β1A-expressing cells adherent to either vitronectin or fibronectin are contracted and exhibit numerous filopodia. These results reveal, therefore, that GD25 cells adherent to vitronectin can only assume a shape suitable for assembly of fibronectin when there is a countervailing signal from functional β1-integrins.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology