Functional alterations of human platelets following 111In labeling with different ligands and incubation media

M. Mieno, Y. Isaka, K. Kimura, M. Matsumoto, H. Etani, A. Uehara, K. Hashikawa, Ryuji Hata, H. Moriwaki, K. Ashida, M. Imaizumi, T. Kamada, T. Kozuka

Research output: Contribution to journalArticle

Abstract

We studied the effects of various 111In-water soluble chelates and incubation media on labeling efficiency of platelets and in vitro platelet aggregability. High labeling efficiency of platelets in ACD-saline was achieved with 111In-oxine sulfate, 111In-tropolone and 111In-MPO (2-mercaptopyridine-N-oxide). In the condition with 4.8 x 106/mm3 platelets in ACD-plasma, 111In-oxine-sulfate had low labeling efficiency and inconsistent labeling, while 111In-tropolone and 111In-MPO had high labeling efficiency. In vitro platelet aggregability (ADP 2 μM) was reduced when platelets were labeled in the absence of plasma. However, there was no significant difference in platelet aggregability among 111In-platelets labeled by three different chelates. In conclusion, to maintain aggregation activity of the platelets with relatively high labeling efficiency, the best result was obtained by using MPO or tropolone chelate in plasma at 4.8 x 106/μl platelet concentration.

Original languageEnglish
Pages (from-to)443-449
Number of pages7
JournalKakuigaku
Volume27
Issue number5
Publication statusPublished - 01-01-1990

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Blood Platelets
Ligands
Tropolone
Sulfates
Platelet Aggregation
Adenosine Diphosphate
Water

All Science Journal Classification (ASJC) codes

  • Radiology Nuclear Medicine and imaging

Cite this

Mieno, M., Isaka, Y., Kimura, K., Matsumoto, M., Etani, H., Uehara, A., ... Kozuka, T. (1990). Functional alterations of human platelets following 111In labeling with different ligands and incubation media. Kakuigaku, 27(5), 443-449.
Mieno, M. ; Isaka, Y. ; Kimura, K. ; Matsumoto, M. ; Etani, H. ; Uehara, A. ; Hashikawa, K. ; Hata, Ryuji ; Moriwaki, H. ; Ashida, K. ; Imaizumi, M. ; Kamada, T. ; Kozuka, T. / Functional alterations of human platelets following 111In labeling with different ligands and incubation media. In: Kakuigaku. 1990 ; Vol. 27, No. 5. pp. 443-449.
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Mieno, M, Isaka, Y, Kimura, K, Matsumoto, M, Etani, H, Uehara, A, Hashikawa, K, Hata, R, Moriwaki, H, Ashida, K, Imaizumi, M, Kamada, T & Kozuka, T 1990, 'Functional alterations of human platelets following 111In labeling with different ligands and incubation media', Kakuigaku, vol. 27, no. 5, pp. 443-449.

Functional alterations of human platelets following 111In labeling with different ligands and incubation media. / Mieno, M.; Isaka, Y.; Kimura, K.; Matsumoto, M.; Etani, H.; Uehara, A.; Hashikawa, K.; Hata, Ryuji; Moriwaki, H.; Ashida, K.; Imaizumi, M.; Kamada, T.; Kozuka, T.

In: Kakuigaku, Vol. 27, No. 5, 01.01.1990, p. 443-449.

Research output: Contribution to journalArticle

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AU - Mieno, M.

AU - Isaka, Y.

AU - Kimura, K.

AU - Matsumoto, M.

AU - Etani, H.

AU - Uehara, A.

AU - Hashikawa, K.

AU - Hata, Ryuji

AU - Moriwaki, H.

AU - Ashida, K.

AU - Imaizumi, M.

AU - Kamada, T.

AU - Kozuka, T.

PY - 1990/1/1

Y1 - 1990/1/1

N2 - We studied the effects of various 111In-water soluble chelates and incubation media on labeling efficiency of platelets and in vitro platelet aggregability. High labeling efficiency of platelets in ACD-saline was achieved with 111In-oxine sulfate, 111In-tropolone and 111In-MPO (2-mercaptopyridine-N-oxide). In the condition with 4.8 x 106/mm3 platelets in ACD-plasma, 111In-oxine-sulfate had low labeling efficiency and inconsistent labeling, while 111In-tropolone and 111In-MPO had high labeling efficiency. In vitro platelet aggregability (ADP 2 μM) was reduced when platelets were labeled in the absence of plasma. However, there was no significant difference in platelet aggregability among 111In-platelets labeled by three different chelates. In conclusion, to maintain aggregation activity of the platelets with relatively high labeling efficiency, the best result was obtained by using MPO or tropolone chelate in plasma at 4.8 x 106/μl platelet concentration.

AB - We studied the effects of various 111In-water soluble chelates and incubation media on labeling efficiency of platelets and in vitro platelet aggregability. High labeling efficiency of platelets in ACD-saline was achieved with 111In-oxine sulfate, 111In-tropolone and 111In-MPO (2-mercaptopyridine-N-oxide). In the condition with 4.8 x 106/mm3 platelets in ACD-plasma, 111In-oxine-sulfate had low labeling efficiency and inconsistent labeling, while 111In-tropolone and 111In-MPO had high labeling efficiency. In vitro platelet aggregability (ADP 2 μM) was reduced when platelets were labeled in the absence of plasma. However, there was no significant difference in platelet aggregability among 111In-platelets labeled by three different chelates. In conclusion, to maintain aggregation activity of the platelets with relatively high labeling efficiency, the best result was obtained by using MPO or tropolone chelate in plasma at 4.8 x 106/μl platelet concentration.

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Mieno M, Isaka Y, Kimura K, Matsumoto M, Etani H, Uehara A et al. Functional alterations of human platelets following 111In labeling with different ligands and incubation media. Kakuigaku. 1990 Jan 1;27(5):443-449.