Functional analysis of the γ-glutamylcysteine synthetase of Escherichia coli B: effect of substitution of His-150 to Ala

Yoshiharu Inoue, Yoshitaka Iba, Hiroshi Yano, Kousaku Murata, Akira Kimura

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

A high expression system of the γ-glutamylcysteine synthetase gene (gshl) of Escherichia coli B was constructed, and rapid purification of GSH-I was performed. The active site of GSH-I was analysed by chemical modification, and Lys, Arg and His residues seemed to be involved in the active site of the enzyme. Among them, His residues were substituted to Ala by site-directed mutagenesis, and His-150 was found to be essential for the activity of GSH-I.

Original languageEnglish
Pages (from-to)473-477
Number of pages5
JournalApplied Microbiology and Biotechnology
Volume38
Issue number4
DOIs
Publication statusPublished - 01-01-1993
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

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