TY - JOUR
T1 - Functional analysis of the γ-glutamylcysteine synthetase of Escherichia coli B
T2 - effect of substitution of His-150 to Ala
AU - Inoue, Yoshiharu
AU - Iba, Yoshitaka
AU - Yano, Hiroshi
AU - Murata, Kousaku
AU - Kimura, Akira
PY - 1993/1
Y1 - 1993/1
N2 - A high expression system of the γ-glutamylcysteine synthetase gene (gshl) of Escherichia coli B was constructed, and rapid purification of GSH-I was performed. The active site of GSH-I was analysed by chemical modification, and Lys, Arg and His residues seemed to be involved in the active site of the enzyme. Among them, His residues were substituted to Ala by site-directed mutagenesis, and His-150 was found to be essential for the activity of GSH-I.
AB - A high expression system of the γ-glutamylcysteine synthetase gene (gshl) of Escherichia coli B was constructed, and rapid purification of GSH-I was performed. The active site of GSH-I was analysed by chemical modification, and Lys, Arg and His residues seemed to be involved in the active site of the enzyme. Among them, His residues were substituted to Ala by site-directed mutagenesis, and His-150 was found to be essential for the activity of GSH-I.
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U2 - 10.1007/BF00242940
DO - 10.1007/BF00242940
M3 - Article
C2 - 7765288
AN - SCOPUS:0027458719
SN - 0175-7598
VL - 38
SP - 473
EP - 477
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 4
ER -