Functional analysis of the stem-loop structures at the 5′ end of the Aichi virus genome

Shigeo Nagashima, Jun Sasaki, Koki Taniguchi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Aichi virus is a member of the family Picornaviridae. Computer-assisted secondary structure prediction suggested the formation of three stem-loop structures (SL-A, SL-B, and SL-C from the 5′ end) within the 5′-end 120 nucleotides of the genome. We have already shown that the most 5′-end stem-loop, SL-A, is critical for viral RNA replication. Here, using an infectious cDNA clone and a replicon harboring a luciferase gene, we revealed that formation of SL-B and SL-C on the positive strand is essential for viral RNA replication. In addition, the specific nucleotide sequence of the loop segment of SL-B was also shown to be critical for viral RNA replication. Mutations of the upper and lower stems of SL-C that do not disrupt the base-pairings hardly affected RNA replication, but decreased the yields of viable viruses significantly compared with for the wild-type. This suggests that SL-C plays a role at some step besides RNA replication during virus infection.

Original languageEnglish
Pages (from-to)56-65
Number of pages10
JournalVirology
Volume313
Issue number1
DOIs
Publication statusPublished - 15-08-2003

All Science Journal Classification (ASJC) codes

  • Virology

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