Functional brain mapping using specific sensory-circuit stimulation and a theoretical graph network analysis in mice with neuropathic allodynia

Yuji Komaki, Keigo Hikishima, Shinsuke Shibata, Tsunehiko Konomi, Fumiko Seki, Masayuki Yamada, Naoyuki Miyasaka, Kanehiro Fujiyoshi, Hirotaka J. Okano, Masaya Nakamura, Hideyuki Okano

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11 Citations (Scopus)

Abstract

Allodynia, a form of neuropathic pain, is defined as pain in response to a non-nociceptive stimulus. The brain regions responsible for pain, which are not normally activated, can be activated in allodynic mice by providing a suitable stimulus to Aβ-fibers, which transmit signals from tactile sensory fibers. Functional MRI (fMRI) can be used to objectively observe abnormal brain activation. In the present study, fMRI was conducted to investigate allodynia in mice; allodynia was generated by surgical injury at the L4 spinal nerve root, thus selectively stimulating sensory nerve fibers. In intact mice, only the primary somatosensory cortex (S1) was activated by stimulation of Aβ-fibers. Meanwhile, allodynic mice showed significantly higher BOLD signals in the anterior cingulate area (ACA) and thalamus. Using resting state fMRI, both degree and eigenvector centrality were significantly decreased in the contralateral S1, clustering coefficient and local efficiency were significantly increased in the ACA, and betweenness centrality was significantly higher in the ventral posterolateral nucleus of the thalamus. These results suggest that the observed abnormal BOLD activation is associated with defects in Aβ-fibers when Aβ-fibers in allodynic mice are selectively stimulated. The objective approach enabled by fMRI can improve our understanding of pathophysiological mechanisms and therapeutic efficacy.

Original languageEnglish
Article number37802
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 29-11-2016

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Brain Mapping
Hyperalgesia
Myelinated Nerve Fibers
Gyrus Cinguli
Magnetic Resonance Imaging
Ventral Thalamic Nuclei
Anterior Thalamic Nuclei
Pain
Somatosensory Cortex
Spinal Nerve Roots
Intraoperative Complications
Brain
Touch
Neuralgia
Nerve Fibers
Cluster Analysis

All Science Journal Classification (ASJC) codes

  • General

Cite this

Komaki, Yuji ; Hikishima, Keigo ; Shibata, Shinsuke ; Konomi, Tsunehiko ; Seki, Fumiko ; Yamada, Masayuki ; Miyasaka, Naoyuki ; Fujiyoshi, Kanehiro ; Okano, Hirotaka J. ; Nakamura, Masaya ; Okano, Hideyuki. / Functional brain mapping using specific sensory-circuit stimulation and a theoretical graph network analysis in mice with neuropathic allodynia. In: Scientific Reports. 2016 ; Vol. 6.
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abstract = "Allodynia, a form of neuropathic pain, is defined as pain in response to a non-nociceptive stimulus. The brain regions responsible for pain, which are not normally activated, can be activated in allodynic mice by providing a suitable stimulus to Aβ-fibers, which transmit signals from tactile sensory fibers. Functional MRI (fMRI) can be used to objectively observe abnormal brain activation. In the present study, fMRI was conducted to investigate allodynia in mice; allodynia was generated by surgical injury at the L4 spinal nerve root, thus selectively stimulating sensory nerve fibers. In intact mice, only the primary somatosensory cortex (S1) was activated by stimulation of Aβ-fibers. Meanwhile, allodynic mice showed significantly higher BOLD signals in the anterior cingulate area (ACA) and thalamus. Using resting state fMRI, both degree and eigenvector centrality were significantly decreased in the contralateral S1, clustering coefficient and local efficiency were significantly increased in the ACA, and betweenness centrality was significantly higher in the ventral posterolateral nucleus of the thalamus. These results suggest that the observed abnormal BOLD activation is associated with defects in Aβ-fibers when Aβ-fibers in allodynic mice are selectively stimulated. The objective approach enabled by fMRI can improve our understanding of pathophysiological mechanisms and therapeutic efficacy.",
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Komaki, Y, Hikishima, K, Shibata, S, Konomi, T, Seki, F, Yamada, M, Miyasaka, N, Fujiyoshi, K, Okano, HJ, Nakamura, M & Okano, H 2016, 'Functional brain mapping using specific sensory-circuit stimulation and a theoretical graph network analysis in mice with neuropathic allodynia', Scientific Reports, vol. 6, 37802. https://doi.org/10.1038/srep37802

Functional brain mapping using specific sensory-circuit stimulation and a theoretical graph network analysis in mice with neuropathic allodynia. / Komaki, Yuji; Hikishima, Keigo; Shibata, Shinsuke; Konomi, Tsunehiko; Seki, Fumiko; Yamada, Masayuki; Miyasaka, Naoyuki; Fujiyoshi, Kanehiro; Okano, Hirotaka J.; Nakamura, Masaya; Okano, Hideyuki.

In: Scientific Reports, Vol. 6, 37802, 29.11.2016.

Research output: Contribution to journalArticle

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AU - Okano, Hideyuki

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