Functional expression of cloned human splicing factor SF2: homology to rna-binding proteins, U1 70K, and drosophila splicing regulators

Adrian R. Krainer, Akira Maeda, Diane Kozak, Georgia Binns

Research output: Contribution to journalArticle

375 Citations (Scopus)

Abstract

SF2 is a protein factor essential for constitutive pre-mRNA splicing in HeLa cell extracts and also activates proximal alternative 5′ splice sites in a concentration dependent manner. This latter property suggests a role for SF2 in preventing axon skipping, ensuring the accuracy of splicing, and regulating alternative splicing. Human SF2 cDNAs have been isolated and overexpressed in bacteria. Recombinant SF2 is active in splicing and stimulates proximal 5′ splice sites. SF2 has a C-terminal region rich in arginine-serine dipeptides, similar to the RS domains of the U1 snRNP 70K polypeptide and the Drosophila alternative splicing regulators transformer, transformer-2, and suppressor-of-white-apricot. Like transformer-2 and 70K, SF2 contains an RNP-type RNA recognition motif.

Original languageEnglish
Pages (from-to)383-394
Number of pages12
JournalCell
Volume66
Issue number2
DOIs
Publication statusPublished - 26-07-1991

Fingerprint

RNA Splice Sites
RNA-Binding Proteins
Alternative Splicing
Drosophila
U1 Small Nuclear Ribonucleoproteins
Dipeptides
RNA Precursors
Serine
Arginine
Bacteria
Complementary DNA
RNA
Cell Extracts
HeLa Cells
Peptides
Axons
Proteins
RNA Splicing Factors

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

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Functional expression of cloned human splicing factor SF2 : homology to rna-binding proteins, U1 70K, and drosophila splicing regulators. / Krainer, Adrian R.; Maeda, Akira; Kozak, Diane; Binns, Georgia.

In: Cell, Vol. 66, No. 2, 26.07.1991, p. 383-394.

Research output: Contribution to journalArticle

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