Functional gene cloning and characterization of the SsmE multidrug efflux pump from Serratia marcescens

Yusuke Minato; Fereshteh Shahcheraghi; Wakano Ogawa; Teruo Kuroda; Tomofusa Tsuchiya

doi:10.1248/bpb.31.516

Functional gene cloning and characterization of the SsmE multidrug efflux pump from Serratia marcescens

Yusuke Minato, Fereshteh Shahcheraghi, Wakano Ogawa, Teruo Kuroda, Tomofusa Tsuchiya

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

We cloned a gene responsible for multidrug resistance from chromosomal DNA of Serratia marcescens, and determined the nucleotide sequence. We designated the gene as ssmE. The deduced amino acid sequence of SsmE showed high similarity with the small multidrug resistance (SMR)-type multidrug efflux pumps. Cells of Escherichia coli KAM32, a drug hyper-susceptible mutant, transformed with a plasmid pESM437 carrying the ssmE gene showed elevated minimum inhibitory concentrations of structurally unrelated antimicrobial agents. E. coli KAM32/pESM437 showed elevated energy dependent efflux of ethidium. Reverse transcriptase polymerase chain reaction (RT-PCR) analysis revealed that ssmE was expressed in cells of S. marcescens.

Original language	English
Pages (from-to)	516-519
Number of pages	4
Journal	Biological and Pharmaceutical Bulletin
Volume	31
Issue number	3
DOIs	https://doi.org/10.1248/bpb.31.516
Publication status	Published - 03-2008
Externally published	Yes

All Science Journal Classification (ASJC) codes

Pharmacology
Pharmaceutical Science

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abstract = "We cloned a gene responsible for multidrug resistance from chromosomal DNA of Serratia marcescens, and determined the nucleotide sequence. We designated the gene as ssmE. The deduced amino acid sequence of SsmE showed high similarity with the small multidrug resistance (SMR)-type multidrug efflux pumps. Cells of Escherichia coli KAM32, a drug hyper-susceptible mutant, transformed with a plasmid pESM437 carrying the ssmE gene showed elevated minimum inhibitory concentrations of structurally unrelated antimicrobial agents. E. coli KAM32/pESM437 showed elevated energy dependent efflux of ethidium. Reverse transcriptase polymerase chain reaction (RT-PCR) analysis revealed that ssmE was expressed in cells of S. marcescens.",

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AU - Minato, Yusuke

AU - Shahcheraghi, Fereshteh

AU - Ogawa, Wakano

AU - Kuroda, Teruo

AU - Tsuchiya, Tomofusa

PY - 2008/3

Y1 - 2008/3

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AB - We cloned a gene responsible for multidrug resistance from chromosomal DNA of Serratia marcescens, and determined the nucleotide sequence. We designated the gene as ssmE. The deduced amino acid sequence of SsmE showed high similarity with the small multidrug resistance (SMR)-type multidrug efflux pumps. Cells of Escherichia coli KAM32, a drug hyper-susceptible mutant, transformed with a plasmid pESM437 carrying the ssmE gene showed elevated minimum inhibitory concentrations of structurally unrelated antimicrobial agents. E. coli KAM32/pESM437 showed elevated energy dependent efflux of ethidium. Reverse transcriptase polymerase chain reaction (RT-PCR) analysis revealed that ssmE was expressed in cells of S. marcescens.

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Functional gene cloning and characterization of the SsmE multidrug efflux pump from Serratia marcescens

Abstract

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