Functional promoter polymorphisms of macrophage migration inhibitory factor in peptic ulcer diseases

Hisakazu Shiroeda, Tomomitsu Tahara, Tomoyuki Shibata, Masakatsu Nakamura, Hideto Yamada, Tomoe Nomura, Ranji Hayashi, Takashi Saito, Tomoki Fukuyama, Toshimi Otsuka, Hirokazu Yano, Kazuaki Ozaki, Mutsumi Tsuchishima, Mikihiro Tsutsumi, Tomiyasu Arisawa

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Macrophage migration inhibitory factor (MIF) is a key proinflammatory mediator, which plays a pivotal role in inflammatory and immune diseases. We attempted to clarify the association of functional polymorphisms of MIF gene promoter with the development of gastro-duodenal ulcer. The study was performed in 471 stocked DNAs obtained from the subjects, including 93 healthy volunteers, with no evidence of gastric malignancy. We employed the PCR-SSCP method to detect gene polymorphisms. In all 471 DNAs, 92 and 43 were obtained from gastric and duodenal ulcer patients, respectively. By an unadjusted analysis, infection with Helicobacter pylori (H. pylori), male gender and non-steroidal anti-inflammatory drug (NSAID/aspirin) use were significantly associated with a risk for developing a gastric ulcer, whereas MIF promoter polymorphisms were not. On the other hand, infection with H. pylori, male gender and 7-CATT repeat at position -794 were significantly associated with the development of a duodenal ulcer, whereas NSAID/ aspirin use was not. By the analysis after adjustment for age, gender, NSAID/aspirin use and H. pylori infection status, 7/7-CATT homozygote had a significantly increased risk for the development of duodenal ulcers (OR, 6.31; 95% CI, 1.50-26.6; p=0.012). No factors were significantly associated with the development of peptic ulcers in NSAID/aspirin users. Our results suggested that tetranucleotide repeat polymorphism of MIF gene promoter might be associated with the development of duodenal ulcers.

Original languageEnglish
Pages (from-to)707-711
Number of pages5
JournalInternational Journal of Molecular Medicine
Volume26
Issue number5
DOIs
Publication statusPublished - 01-11-2010

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Macrophage Migration-Inhibitory Factors
Duodenal Ulcer
Peptic Ulcer
Non-Steroidal Anti-Inflammatory Agents
Aspirin
Helicobacter pylori
Stomach Ulcer
Genes
Single-Stranded Conformational Polymorphism
DNA
Immune System Diseases
Homozygote
Helicobacter Infections
Infection
Microsatellite Repeats
Stomach
Healthy Volunteers
Anti-Inflammatory Agents
Polymerase Chain Reaction
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Shiroeda, Hisakazu ; Tahara, Tomomitsu ; Shibata, Tomoyuki ; Nakamura, Masakatsu ; Yamada, Hideto ; Nomura, Tomoe ; Hayashi, Ranji ; Saito, Takashi ; Fukuyama, Tomoki ; Otsuka, Toshimi ; Yano, Hirokazu ; Ozaki, Kazuaki ; Tsuchishima, Mutsumi ; Tsutsumi, Mikihiro ; Arisawa, Tomiyasu. / Functional promoter polymorphisms of macrophage migration inhibitory factor in peptic ulcer diseases. In: International Journal of Molecular Medicine. 2010 ; Vol. 26, No. 5. pp. 707-711.
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abstract = "Macrophage migration inhibitory factor (MIF) is a key proinflammatory mediator, which plays a pivotal role in inflammatory and immune diseases. We attempted to clarify the association of functional polymorphisms of MIF gene promoter with the development of gastro-duodenal ulcer. The study was performed in 471 stocked DNAs obtained from the subjects, including 93 healthy volunteers, with no evidence of gastric malignancy. We employed the PCR-SSCP method to detect gene polymorphisms. In all 471 DNAs, 92 and 43 were obtained from gastric and duodenal ulcer patients, respectively. By an unadjusted analysis, infection with Helicobacter pylori (H. pylori), male gender and non-steroidal anti-inflammatory drug (NSAID/aspirin) use were significantly associated with a risk for developing a gastric ulcer, whereas MIF promoter polymorphisms were not. On the other hand, infection with H. pylori, male gender and 7-CATT repeat at position -794 were significantly associated with the development of a duodenal ulcer, whereas NSAID/ aspirin use was not. By the analysis after adjustment for age, gender, NSAID/aspirin use and H. pylori infection status, 7/7-CATT homozygote had a significantly increased risk for the development of duodenal ulcers (OR, 6.31; 95{\%} CI, 1.50-26.6; p=0.012). No factors were significantly associated with the development of peptic ulcers in NSAID/aspirin users. Our results suggested that tetranucleotide repeat polymorphism of MIF gene promoter might be associated with the development of duodenal ulcers.",
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Shiroeda, H, Tahara, T, Shibata, T, Nakamura, M, Yamada, H, Nomura, T, Hayashi, R, Saito, T, Fukuyama, T, Otsuka, T, Yano, H, Ozaki, K, Tsuchishima, M, Tsutsumi, M & Arisawa, T 2010, 'Functional promoter polymorphisms of macrophage migration inhibitory factor in peptic ulcer diseases', International Journal of Molecular Medicine, vol. 26, no. 5, pp. 707-711. https://doi.org/10.3892/ijmm-00000517

Functional promoter polymorphisms of macrophage migration inhibitory factor in peptic ulcer diseases. / Shiroeda, Hisakazu; Tahara, Tomomitsu; Shibata, Tomoyuki; Nakamura, Masakatsu; Yamada, Hideto; Nomura, Tomoe; Hayashi, Ranji; Saito, Takashi; Fukuyama, Tomoki; Otsuka, Toshimi; Yano, Hirokazu; Ozaki, Kazuaki; Tsuchishima, Mutsumi; Tsutsumi, Mikihiro; Arisawa, Tomiyasu.

In: International Journal of Molecular Medicine, Vol. 26, No. 5, 01.11.2010, p. 707-711.

Research output: Contribution to journalArticle

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T1 - Functional promoter polymorphisms of macrophage migration inhibitory factor in peptic ulcer diseases

AU - Shiroeda, Hisakazu

AU - Tahara, Tomomitsu

AU - Shibata, Tomoyuki

AU - Nakamura, Masakatsu

AU - Yamada, Hideto

AU - Nomura, Tomoe

AU - Hayashi, Ranji

AU - Saito, Takashi

AU - Fukuyama, Tomoki

AU - Otsuka, Toshimi

AU - Yano, Hirokazu

AU - Ozaki, Kazuaki

AU - Tsuchishima, Mutsumi

AU - Tsutsumi, Mikihiro

AU - Arisawa, Tomiyasu

PY - 2010/11/1

Y1 - 2010/11/1

N2 - Macrophage migration inhibitory factor (MIF) is a key proinflammatory mediator, which plays a pivotal role in inflammatory and immune diseases. We attempted to clarify the association of functional polymorphisms of MIF gene promoter with the development of gastro-duodenal ulcer. The study was performed in 471 stocked DNAs obtained from the subjects, including 93 healthy volunteers, with no evidence of gastric malignancy. We employed the PCR-SSCP method to detect gene polymorphisms. In all 471 DNAs, 92 and 43 were obtained from gastric and duodenal ulcer patients, respectively. By an unadjusted analysis, infection with Helicobacter pylori (H. pylori), male gender and non-steroidal anti-inflammatory drug (NSAID/aspirin) use were significantly associated with a risk for developing a gastric ulcer, whereas MIF promoter polymorphisms were not. On the other hand, infection with H. pylori, male gender and 7-CATT repeat at position -794 were significantly associated with the development of a duodenal ulcer, whereas NSAID/ aspirin use was not. By the analysis after adjustment for age, gender, NSAID/aspirin use and H. pylori infection status, 7/7-CATT homozygote had a significantly increased risk for the development of duodenal ulcers (OR, 6.31; 95% CI, 1.50-26.6; p=0.012). No factors were significantly associated with the development of peptic ulcers in NSAID/aspirin users. Our results suggested that tetranucleotide repeat polymorphism of MIF gene promoter might be associated with the development of duodenal ulcers.

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