Functional SNP in an Sp1-binding site of AGTRL1 gene is associated with susceptibility to brain infarction

Jun Hata, Koichi Matsuda, Toshiharu Ninomiya, Koji Yonemoto, Tomonaga Matsushita, Yozo Ohnishi, Susumu Saito, Takanari Kitazono, Setsuro Ibayashi, Mitsuo Iida, Yutaka Kiyohara, Yusuke Nakamura, Michiaki Kubo

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Abstract

Brain infarction is one of the common causes of death and also a major cause of severe disability. To identify a gene(s) susceptible to brain infarction, we performed a large-scale association study of Japanese patients with brain infarction, using 52608 gene-based single nucleotide polymorphism (SNP) markers. Comparison of allele frequencies between 1112 cases with brain infarction and age- and sex-matched control subjects of the same number found an SNP in the 5′-flanking region of angiotensin receptor like-1 (AGTRL1) gene (rs9943582, -154G/A) to have a significant association with brain infarction [odds ratio = 1.30, 95% confidence interval (CI) = 1.14-1.47, P = 0.000066]. We also found the binding of Sp1 transcription factor to the region including the susceptible G allele, but not the non-susceptible A allele. Luciferase assay and RT-PCR analysis demonstrated that exogenously introduced Sp1 induced transcription of AGTRL1 and its ligand, apelin, as well, indicating direct regulation of apelin/APJ pathway by Sp1. Furthermore, a 14 year follow-up cohort study in a Japanese community in Hisayama town, Japan revealed that the homozygote of the susceptible G allele of this particular SNP had significantly higher risk of brain infarction (hazard ratio = 2.00, 95% CI = 1.22-3.29, P = 0.006). Our results indicate that the SNP in the AGTRL1 gene is associated with the susceptibility to brain infarction.

Original languageEnglish
Pages (from-to)630-639
Number of pages10
JournalHuman molecular genetics
Volume16
Issue number6
DOIs
Publication statusPublished - 15-03-2007

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Brain Infarction
Angiotensin Receptors
Single Nucleotide Polymorphism
Binding Sites
Genes
Alleles
Confidence Intervals
Sp1 Transcription Factor
5' Flanking Region
Homozygote
Luciferases
Gene Frequency
Cause of Death
Japan
Cohort Studies
Odds Ratio
Ligands
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Hata, Jun ; Matsuda, Koichi ; Ninomiya, Toshiharu ; Yonemoto, Koji ; Matsushita, Tomonaga ; Ohnishi, Yozo ; Saito, Susumu ; Kitazono, Takanari ; Ibayashi, Setsuro ; Iida, Mitsuo ; Kiyohara, Yutaka ; Nakamura, Yusuke ; Kubo, Michiaki. / Functional SNP in an Sp1-binding site of AGTRL1 gene is associated with susceptibility to brain infarction. In: Human molecular genetics. 2007 ; Vol. 16, No. 6. pp. 630-639.
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abstract = "Brain infarction is one of the common causes of death and also a major cause of severe disability. To identify a gene(s) susceptible to brain infarction, we performed a large-scale association study of Japanese patients with brain infarction, using 52608 gene-based single nucleotide polymorphism (SNP) markers. Comparison of allele frequencies between 1112 cases with brain infarction and age- and sex-matched control subjects of the same number found an SNP in the 5′-flanking region of angiotensin receptor like-1 (AGTRL1) gene (rs9943582, -154G/A) to have a significant association with brain infarction [odds ratio = 1.30, 95{\%} confidence interval (CI) = 1.14-1.47, P = 0.000066]. We also found the binding of Sp1 transcription factor to the region including the susceptible G allele, but not the non-susceptible A allele. Luciferase assay and RT-PCR analysis demonstrated that exogenously introduced Sp1 induced transcription of AGTRL1 and its ligand, apelin, as well, indicating direct regulation of apelin/APJ pathway by Sp1. Furthermore, a 14 year follow-up cohort study in a Japanese community in Hisayama town, Japan revealed that the homozygote of the susceptible G allele of this particular SNP had significantly higher risk of brain infarction (hazard ratio = 2.00, 95{\%} CI = 1.22-3.29, P = 0.006). Our results indicate that the SNP in the AGTRL1 gene is associated with the susceptibility to brain infarction.",
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Hata, J, Matsuda, K, Ninomiya, T, Yonemoto, K, Matsushita, T, Ohnishi, Y, Saito, S, Kitazono, T, Ibayashi, S, Iida, M, Kiyohara, Y, Nakamura, Y & Kubo, M 2007, 'Functional SNP in an Sp1-binding site of AGTRL1 gene is associated with susceptibility to brain infarction', Human molecular genetics, vol. 16, no. 6, pp. 630-639. https://doi.org/10.1093/hmg/ddm005

Functional SNP in an Sp1-binding site of AGTRL1 gene is associated with susceptibility to brain infarction. / Hata, Jun; Matsuda, Koichi; Ninomiya, Toshiharu; Yonemoto, Koji; Matsushita, Tomonaga; Ohnishi, Yozo; Saito, Susumu; Kitazono, Takanari; Ibayashi, Setsuro; Iida, Mitsuo; Kiyohara, Yutaka; Nakamura, Yusuke; Kubo, Michiaki.

In: Human molecular genetics, Vol. 16, No. 6, 15.03.2007, p. 630-639.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Functional SNP in an Sp1-binding site of AGTRL1 gene is associated with susceptibility to brain infarction

AU - Hata, Jun

AU - Matsuda, Koichi

AU - Ninomiya, Toshiharu

AU - Yonemoto, Koji

AU - Matsushita, Tomonaga

AU - Ohnishi, Yozo

AU - Saito, Susumu

AU - Kitazono, Takanari

AU - Ibayashi, Setsuro

AU - Iida, Mitsuo

AU - Kiyohara, Yutaka

AU - Nakamura, Yusuke

AU - Kubo, Michiaki

PY - 2007/3/15

Y1 - 2007/3/15

N2 - Brain infarction is one of the common causes of death and also a major cause of severe disability. To identify a gene(s) susceptible to brain infarction, we performed a large-scale association study of Japanese patients with brain infarction, using 52608 gene-based single nucleotide polymorphism (SNP) markers. Comparison of allele frequencies between 1112 cases with brain infarction and age- and sex-matched control subjects of the same number found an SNP in the 5′-flanking region of angiotensin receptor like-1 (AGTRL1) gene (rs9943582, -154G/A) to have a significant association with brain infarction [odds ratio = 1.30, 95% confidence interval (CI) = 1.14-1.47, P = 0.000066]. We also found the binding of Sp1 transcription factor to the region including the susceptible G allele, but not the non-susceptible A allele. Luciferase assay and RT-PCR analysis demonstrated that exogenously introduced Sp1 induced transcription of AGTRL1 and its ligand, apelin, as well, indicating direct regulation of apelin/APJ pathway by Sp1. Furthermore, a 14 year follow-up cohort study in a Japanese community in Hisayama town, Japan revealed that the homozygote of the susceptible G allele of this particular SNP had significantly higher risk of brain infarction (hazard ratio = 2.00, 95% CI = 1.22-3.29, P = 0.006). Our results indicate that the SNP in the AGTRL1 gene is associated with the susceptibility to brain infarction.

AB - Brain infarction is one of the common causes of death and also a major cause of severe disability. To identify a gene(s) susceptible to brain infarction, we performed a large-scale association study of Japanese patients with brain infarction, using 52608 gene-based single nucleotide polymorphism (SNP) markers. Comparison of allele frequencies between 1112 cases with brain infarction and age- and sex-matched control subjects of the same number found an SNP in the 5′-flanking region of angiotensin receptor like-1 (AGTRL1) gene (rs9943582, -154G/A) to have a significant association with brain infarction [odds ratio = 1.30, 95% confidence interval (CI) = 1.14-1.47, P = 0.000066]. We also found the binding of Sp1 transcription factor to the region including the susceptible G allele, but not the non-susceptible A allele. Luciferase assay and RT-PCR analysis demonstrated that exogenously introduced Sp1 induced transcription of AGTRL1 and its ligand, apelin, as well, indicating direct regulation of apelin/APJ pathway by Sp1. Furthermore, a 14 year follow-up cohort study in a Japanese community in Hisayama town, Japan revealed that the homozygote of the susceptible G allele of this particular SNP had significantly higher risk of brain infarction (hazard ratio = 2.00, 95% CI = 1.22-3.29, P = 0.006). Our results indicate that the SNP in the AGTRL1 gene is associated with the susceptibility to brain infarction.

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