Fusobacterium Detected in Colonic Biopsy and Clinicopathological Features of Ulcerative Colitis in Japan

Tomomitsu Tahara, Tomoyuki Shibata, Tomohiko Kawamura, Masaaki Okubo, Yuichiro Ichikawa, Kazuya Sumi, Masahiro Miyata, Takamitsu Ishizuka, Masakatsu Nakamura, Mitsuo Nagasaka, Yoshihito Nakagawa, Naoki Ohmiya, Tomiyasu Arisawa, Ichiro Hirata

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background and Aims: Fusobacterium species are part of the gut microbiome in humans, but some species have been recognized as opportunistic pathogens implicated in inflammatory diseases including inflammatory bowel diseases. Here, we performed prevalence screening of Fusobacterium in ulcerative colitis (UC) in Japanese patients.

Methods: We examined Fusobacterium nucleatum (F. nucleatum) and whole Fusobacteriumspecies (Pan-fusobacterium) by quantitative real-time PCR in 163 inflamed mucosae from 152 UC patients. Data were correlated with clinical subtypes of UC.

Results: In an initial prevalence screen, F. nucleatum and Pan-fusobacterium were detected in 6.3 % (4/64) and 53.1 % (34/64). For all 163 mucosae, the prevalence of Pan-fusobacterium was 54.6 % (89/163). Pan-fusobacterium status was concordant in inflamed and normal adjacent samples, and the matched cases during 1-year follow-up colonoscopy. The higher amount of Pan-fusobacterium was observed in chronic continuous type compared to one attack and relapse/remitting type (p = 0.039). The higher amount of Pan-fusobacterium was also associated with rather mild clinical course of disease, such as non-steroid dependency (p = 0.015), non-refractory phenotype (p = 0.013), and non-severe phenotype (p = 0.04). Based on the distribution of Pan-fusobacterium measurable cases, we identified 10 cases as having a high amount of Pan-fusobacterium (FB-high). The clinicopathological features of FB-high UC cases were also highlighted by chronic continuous type and mild phenotypes of disease.

Conclusion: Whole Fusobacterium species, but not F. nucleatum, are common in UC patients and have a role in persistence of colonic inflammation in UC. However, Fusobacterium infection is associated with rather mild clinical phenotypes of UC.

Original languageEnglish
Pages (from-to)205-210
Number of pages6
JournalDigestive Diseases and Sciences
Volume60
Issue number1
DOIs
Publication statusPublished - 01-01-2014

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Fusobacterium
Ulcerative Colitis
Japan
Biopsy
Fusobacterium nucleatum
Phenotype
Fusobacterium Infections
Mucous Membrane
Colonoscopy
Inflammatory Bowel Diseases
Real-Time Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Physiology
  • Gastroenterology

Cite this

Tahara, Tomomitsu ; Shibata, Tomoyuki ; Kawamura, Tomohiko ; Okubo, Masaaki ; Ichikawa, Yuichiro ; Sumi, Kazuya ; Miyata, Masahiro ; Ishizuka, Takamitsu ; Nakamura, Masakatsu ; Nagasaka, Mitsuo ; Nakagawa, Yoshihito ; Ohmiya, Naoki ; Arisawa, Tomiyasu ; Hirata, Ichiro. / Fusobacterium Detected in Colonic Biopsy and Clinicopathological Features of Ulcerative Colitis in Japan. In: Digestive Diseases and Sciences. 2014 ; Vol. 60, No. 1. pp. 205-210.
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title = "Fusobacterium Detected in Colonic Biopsy and Clinicopathological Features of Ulcerative Colitis in Japan",
abstract = "Background and Aims: Fusobacterium species are part of the gut microbiome in humans, but some species have been recognized as opportunistic pathogens implicated in inflammatory diseases including inflammatory bowel diseases. Here, we performed prevalence screening of Fusobacterium in ulcerative colitis (UC) in Japanese patients.Methods: We examined Fusobacterium nucleatum (F. nucleatum) and whole Fusobacteriumspecies (Pan-fusobacterium) by quantitative real-time PCR in 163 inflamed mucosae from 152 UC patients. Data were correlated with clinical subtypes of UC.Results: In an initial prevalence screen, F. nucleatum and Pan-fusobacterium were detected in 6.3 {\%} (4/64) and 53.1 {\%} (34/64). For all 163 mucosae, the prevalence of Pan-fusobacterium was 54.6 {\%} (89/163). Pan-fusobacterium status was concordant in inflamed and normal adjacent samples, and the matched cases during 1-year follow-up colonoscopy. The higher amount of Pan-fusobacterium was observed in chronic continuous type compared to one attack and relapse/remitting type (p = 0.039). The higher amount of Pan-fusobacterium was also associated with rather mild clinical course of disease, such as non-steroid dependency (p = 0.015), non-refractory phenotype (p = 0.013), and non-severe phenotype (p = 0.04). Based on the distribution of Pan-fusobacterium measurable cases, we identified 10 cases as having a high amount of Pan-fusobacterium (FB-high). The clinicopathological features of FB-high UC cases were also highlighted by chronic continuous type and mild phenotypes of disease.Conclusion: Whole Fusobacterium species, but not F. nucleatum, are common in UC patients and have a role in persistence of colonic inflammation in UC. However, Fusobacterium infection is associated with rather mild clinical phenotypes of UC.",
author = "Tomomitsu Tahara and Tomoyuki Shibata and Tomohiko Kawamura and Masaaki Okubo and Yuichiro Ichikawa and Kazuya Sumi and Masahiro Miyata and Takamitsu Ishizuka and Masakatsu Nakamura and Mitsuo Nagasaka and Yoshihito Nakagawa and Naoki Ohmiya and Tomiyasu Arisawa and Ichiro Hirata",
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Tahara, T, Shibata, T, Kawamura, T, Okubo, M, Ichikawa, Y, Sumi, K, Miyata, M, Ishizuka, T, Nakamura, M, Nagasaka, M, Nakagawa, Y, Ohmiya, N, Arisawa, T & Hirata, I 2014, ' Fusobacterium Detected in Colonic Biopsy and Clinicopathological Features of Ulcerative Colitis in Japan', Digestive Diseases and Sciences, vol. 60, no. 1, pp. 205-210. https://doi.org/10.1007/s10620-014-3316-y

Fusobacterium Detected in Colonic Biopsy and Clinicopathological Features of Ulcerative Colitis in Japan. / Tahara, Tomomitsu; Shibata, Tomoyuki; Kawamura, Tomohiko; Okubo, Masaaki; Ichikawa, Yuichiro; Sumi, Kazuya; Miyata, Masahiro; Ishizuka, Takamitsu; Nakamura, Masakatsu; Nagasaka, Mitsuo; Nakagawa, Yoshihito; Ohmiya, Naoki; Arisawa, Tomiyasu; Hirata, Ichiro.

In: Digestive Diseases and Sciences, Vol. 60, No. 1, 01.01.2014, p. 205-210.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Fusobacterium Detected in Colonic Biopsy and Clinicopathological Features of Ulcerative Colitis in Japan

AU - Tahara, Tomomitsu

AU - Shibata, Tomoyuki

AU - Kawamura, Tomohiko

AU - Okubo, Masaaki

AU - Ichikawa, Yuichiro

AU - Sumi, Kazuya

AU - Miyata, Masahiro

AU - Ishizuka, Takamitsu

AU - Nakamura, Masakatsu

AU - Nagasaka, Mitsuo

AU - Nakagawa, Yoshihito

AU - Ohmiya, Naoki

AU - Arisawa, Tomiyasu

AU - Hirata, Ichiro

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Background and Aims: Fusobacterium species are part of the gut microbiome in humans, but some species have been recognized as opportunistic pathogens implicated in inflammatory diseases including inflammatory bowel diseases. Here, we performed prevalence screening of Fusobacterium in ulcerative colitis (UC) in Japanese patients.Methods: We examined Fusobacterium nucleatum (F. nucleatum) and whole Fusobacteriumspecies (Pan-fusobacterium) by quantitative real-time PCR in 163 inflamed mucosae from 152 UC patients. Data were correlated with clinical subtypes of UC.Results: In an initial prevalence screen, F. nucleatum and Pan-fusobacterium were detected in 6.3 % (4/64) and 53.1 % (34/64). For all 163 mucosae, the prevalence of Pan-fusobacterium was 54.6 % (89/163). Pan-fusobacterium status was concordant in inflamed and normal adjacent samples, and the matched cases during 1-year follow-up colonoscopy. The higher amount of Pan-fusobacterium was observed in chronic continuous type compared to one attack and relapse/remitting type (p = 0.039). The higher amount of Pan-fusobacterium was also associated with rather mild clinical course of disease, such as non-steroid dependency (p = 0.015), non-refractory phenotype (p = 0.013), and non-severe phenotype (p = 0.04). Based on the distribution of Pan-fusobacterium measurable cases, we identified 10 cases as having a high amount of Pan-fusobacterium (FB-high). The clinicopathological features of FB-high UC cases were also highlighted by chronic continuous type and mild phenotypes of disease.Conclusion: Whole Fusobacterium species, but not F. nucleatum, are common in UC patients and have a role in persistence of colonic inflammation in UC. However, Fusobacterium infection is associated with rather mild clinical phenotypes of UC.

AB - Background and Aims: Fusobacterium species are part of the gut microbiome in humans, but some species have been recognized as opportunistic pathogens implicated in inflammatory diseases including inflammatory bowel diseases. Here, we performed prevalence screening of Fusobacterium in ulcerative colitis (UC) in Japanese patients.Methods: We examined Fusobacterium nucleatum (F. nucleatum) and whole Fusobacteriumspecies (Pan-fusobacterium) by quantitative real-time PCR in 163 inflamed mucosae from 152 UC patients. Data were correlated with clinical subtypes of UC.Results: In an initial prevalence screen, F. nucleatum and Pan-fusobacterium were detected in 6.3 % (4/64) and 53.1 % (34/64). For all 163 mucosae, the prevalence of Pan-fusobacterium was 54.6 % (89/163). Pan-fusobacterium status was concordant in inflamed and normal adjacent samples, and the matched cases during 1-year follow-up colonoscopy. The higher amount of Pan-fusobacterium was observed in chronic continuous type compared to one attack and relapse/remitting type (p = 0.039). The higher amount of Pan-fusobacterium was also associated with rather mild clinical course of disease, such as non-steroid dependency (p = 0.015), non-refractory phenotype (p = 0.013), and non-severe phenotype (p = 0.04). Based on the distribution of Pan-fusobacterium measurable cases, we identified 10 cases as having a high amount of Pan-fusobacterium (FB-high). The clinicopathological features of FB-high UC cases were also highlighted by chronic continuous type and mild phenotypes of disease.Conclusion: Whole Fusobacterium species, but not F. nucleatum, are common in UC patients and have a role in persistence of colonic inflammation in UC. However, Fusobacterium infection is associated with rather mild clinical phenotypes of UC.

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