Gamma interferon-induced nitric oxide production in mouse CD5+ B1-like cell line and its association with apoptotic cell death

Naoki Koide, Tsuyoshi Sugiyama, Mya Mya Mu, Isamu Mori, Tomoaki Yoshida, Teruaki Hamano, Takashi Yokochi

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

The in vitro effect of gamma interferon (IFN-γ) on nitric oxide (NO) production in a mouse CD5+ B1-like cell line, TH2.52, was studied. The TH2.52 cell line is the hybridoma line between mouse B lymphoma line and mouse splenic B cells and expresses a series of B1 markers. IFN-γ induced a marked NO production in TH2.52 cells through the expression of an inducible type of NO synthase (iNOS). IFN-γ induced NO production was triggered by the Janus tyrosine kinase (JAK)/signal transducer and activator of transcription (STAT) pathway since it was inhibited by AG490, a JAK2 inhibitor. The growth of TH2.52 cells significantly was inhibited in the presence of IFN-γ. A significant number of cells underwent apoptotic cell death, accompanied by the DNA fragmentation, annexin V binding, and caspase 3 activation. N(G)-monomethyl-L-arginine, an iNOS inhibitor, prevented IFN-γ-induced cell death. Therefore, IFN-γ-induced NO production was possible in causing cell death in TH2.52 cells. Further, IFN-γ-induced NO production and cell death significantly were prevented by interleukin-4, a representative Th2 cytokine. The immunological significance of IFN-γ-induced NO production in a mouse B1-like cell line is discussed.

Original languageEnglish
Pages (from-to)669-679
Number of pages11
JournalMICROBIOLOGY and IMMUNOLOGY
Volume47
Issue number9
DOIs
Publication statusPublished - 01-01-2003
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

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