GATA2 zinc finger 2 mutation found in acute myeloid leukemia impairs myeloid differentiation

Keiko Niimi, Hitoshi Kiyoi, Yuichi Ishikawa, Fumihiko Hayakawa, Shingo Kurahashi, Rika Kihara, Akihiro Tomita, Tomoki Naoe

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We identified two novel GATA2 mutations in acute myeloid leukemia (AML). One mutation (p.R308P-GATA2) was a R308P substitution within the zinc finger (ZF)-1 domain, and the other (p.A350_N351ins8-GATA2) was an eight-amino-acid insertion between A350 and N351 residues within the ZF-2 domain. p.R308P-GATA2 did not affect DNA-binding and transcriptional activities, while p.A350_N351ins8-GATA2 reduced them, and impaired G-CSF-induced granulocytic differentiation of 32D cells. Although p.A350_N351ins8-GATA2 did not show a dominant-negative effect over wild-type (Wt)-GATA2 by the reporter assay, it might be involved in the pathophysiology of AML by impairing myeloid differentiation because of little Wt-GATA2 expression in primary AML cells harboring the p.A350_N351ins8 mutation.

Original languageEnglish
Pages (from-to)21-25
Number of pages5
JournalLeukemia Research Reports
Volume2
Issue number1
DOIs
Publication statusPublished - 04-06-2013
Externally publishedYes

Fingerprint

Zinc Fingers
Acute Myeloid Leukemia
Mutation
Granulocyte Colony-Stimulating Factor
Myeloid Cells
Cell Differentiation
Amino Acids
DNA

All Science Journal Classification (ASJC) codes

  • Hematology
  • Oncology

Cite this

Niimi, K., Kiyoi, H., Ishikawa, Y., Hayakawa, F., Kurahashi, S., Kihara, R., ... Naoe, T. (2013). GATA2 zinc finger 2 mutation found in acute myeloid leukemia impairs myeloid differentiation. Leukemia Research Reports, 2(1), 21-25. https://doi.org/10.1016/j.lrr.2013.02.002
Niimi, Keiko ; Kiyoi, Hitoshi ; Ishikawa, Yuichi ; Hayakawa, Fumihiko ; Kurahashi, Shingo ; Kihara, Rika ; Tomita, Akihiro ; Naoe, Tomoki. / GATA2 zinc finger 2 mutation found in acute myeloid leukemia impairs myeloid differentiation. In: Leukemia Research Reports. 2013 ; Vol. 2, No. 1. pp. 21-25.
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Niimi, K, Kiyoi, H, Ishikawa, Y, Hayakawa, F, Kurahashi, S, Kihara, R, Tomita, A & Naoe, T 2013, 'GATA2 zinc finger 2 mutation found in acute myeloid leukemia impairs myeloid differentiation', Leukemia Research Reports, vol. 2, no. 1, pp. 21-25. https://doi.org/10.1016/j.lrr.2013.02.002

GATA2 zinc finger 2 mutation found in acute myeloid leukemia impairs myeloid differentiation. / Niimi, Keiko; Kiyoi, Hitoshi; Ishikawa, Yuichi; Hayakawa, Fumihiko; Kurahashi, Shingo; Kihara, Rika; Tomita, Akihiro; Naoe, Tomoki.

In: Leukemia Research Reports, Vol. 2, No. 1, 04.06.2013, p. 21-25.

Research output: Contribution to journalArticle

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AU - Niimi, Keiko

AU - Kiyoi, Hitoshi

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AU - Kurahashi, Shingo

AU - Kihara, Rika

AU - Tomita, Akihiro

AU - Naoe, Tomoki

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