General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer

Q. Sun, A. Mayeda, R. K. Hampson, A. R. Krainer, F. M. Rottman

Research output: Contribution to journalArticle

241 Citations (Scopus)

Abstract

The general splicing factor SF2/ASF binds in a sequence-specific manner to a purine-rich exonic splicing enhancer (ESE) in the last exon of bovine growth hormone (bGH) pre-mRNA. More importantly, SF2/ASF stimulates in vitro splicing of bGH intron D through specific interaction with the ESE sequences. However, another general splicing factor, SC35, does not bind the ESE sequences and has no effect on bGH intron D splicing. Thus, one possible function of SF2/ASF in alternative and, perhaps, constitutive pre-mRNA splicing is to recognize ESE sequences. The stimulation of bGH intron D splicing by SF2/ASF is counteracted by the addition of hnRNP A1. The relative levels of SF2/ASF and hnRNP A1 influence the efficiency of bGH intron D splicing in vitro and may be the underlying mechanism of this alternative pre-mRNA processing event in vivo.

Original languageEnglish
Pages (from-to)2598-2608
Number of pages11
JournalGenes and Development
Volume7
Issue number12 B
DOIs
Publication statusPublished - 1993

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

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