General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer

Q. Sun, A. Mayeda, R. K. Hampson, A. R. Krainer, F. M. Rottman

Research output: Contribution to journalArticle

235 Citations (Scopus)

Abstract

The general splicing factor SF2/ASF binds in a sequence-specific manner to a purine-rich exonic splicing enhancer (ESE) in the last exon of bovine growth hormone (bGH) pre-mRNA. More importantly, SF2/ASF stimulates in vitro splicing of bGH intron D through specific interaction with the ESE sequences. However, another general splicing factor, SC35, does not bind the ESE sequences and has no effect on bGH intron D splicing. Thus, one possible function of SF2/ASF in alternative and, perhaps, constitutive pre-mRNA splicing is to recognize ESE sequences. The stimulation of bGH intron D splicing by SF2/ASF is counteracted by the addition of hnRNP A1. The relative levels of SF2/ASF and hnRNP A1 influence the efficiency of bGH intron D splicing in vitro and may be the underlying mechanism of this alternative pre-mRNA processing event in vivo.

Original languageEnglish
Pages (from-to)2598-2608
Number of pages11
JournalGenes and Development
Volume7
Issue number12 B
Publication statusPublished - 01-12-1993

Fingerprint

Alternative Splicing
Introns
RNA Precursors
Exons
RNA Splicing Factors
bovine growth hormone
In Vitro Techniques
hnRNP A1

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

Cite this

Sun, Q. ; Mayeda, A. ; Hampson, R. K. ; Krainer, A. R. ; Rottman, F. M. / General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer. In: Genes and Development. 1993 ; Vol. 7, No. 12 B. pp. 2598-2608.
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Sun, Q, Mayeda, A, Hampson, RK, Krainer, AR & Rottman, FM 1993, 'General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer', Genes and Development, vol. 7, no. 12 B, pp. 2598-2608.

General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer. / Sun, Q.; Mayeda, A.; Hampson, R. K.; Krainer, A. R.; Rottman, F. M.

In: Genes and Development, Vol. 7, No. 12 B, 01.12.1993, p. 2598-2608.

Research output: Contribution to journalArticle

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AU - Sun, Q.

AU - Mayeda, A.

AU - Hampson, R. K.

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AB - The general splicing factor SF2/ASF binds in a sequence-specific manner to a purine-rich exonic splicing enhancer (ESE) in the last exon of bovine growth hormone (bGH) pre-mRNA. More importantly, SF2/ASF stimulates in vitro splicing of bGH intron D through specific interaction with the ESE sequences. However, another general splicing factor, SC35, does not bind the ESE sequences and has no effect on bGH intron D splicing. Thus, one possible function of SF2/ASF in alternative and, perhaps, constitutive pre-mRNA splicing is to recognize ESE sequences. The stimulation of bGH intron D splicing by SF2/ASF is counteracted by the addition of hnRNP A1. The relative levels of SF2/ASF and hnRNP A1 influence the efficiency of bGH intron D splicing in vitro and may be the underlying mechanism of this alternative pre-mRNA processing event in vivo.

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