Generation of dopaminergic neurons and pigmented epithelia from primate ES cells by stromal cell-derived inducing activity

Hiroshi Kawasaki, Hirofumi Suemori, Kenji Mizuseki, Kiichi Watanabe, Fumi Urano, Hiroshi Ichinose, Masatoshi Haruta, Masayo Takahashi, Kanako Yoshikawa, Shin Ichi Nishikawa, Norio Nakatsuji, Yoshiki Sasai

Research output: Contribution to journalArticlepeer-review

408 Citations (Scopus)

Abstract

We previously identified a stromal cell-derived inducing activity (SDIA), which induces differentiation of neural cells, including midbrain tyrosine hydroxylase-positive (TH+) dopaminergic neurons, from mouse embryonic stem cells. We report here that SDIA induces efficient neural differentiation also in primate embryonic stem cells. Induced neurons contain TH+ neurons at a frequency of 35% and produce a significant amount of dopamine. Interestingly, differentiation of TH+ neurons from undifferentiated embryonic cells occurs much faster in vitro (10 days) than it does in the embryo (≈5 weeks). In addition, 8% of the colonies contain large patches of Pax6+-pigmented epithelium of the retina. The SDIA method provides an unlimited source of primate cells for the study of pathogenesis, drug development, and transplantation in degenerative diseases such as Parkinson's disease and retinitis pigmentosa.

Original languageEnglish
Pages (from-to)1580-1585
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number3
DOIs
Publication statusPublished - 05-02-2002
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General

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