TY - JOUR
T1 - Generation of dopaminergic neurons and pigmented epithelia from primate ES cells by stromal cell-derived inducing activity
AU - Kawasaki, Hiroshi
AU - Suemori, Hirofumi
AU - Mizuseki, Kenji
AU - Watanabe, Kiichi
AU - Urano, Fumi
AU - Ichinose, Hiroshi
AU - Haruta, Masatoshi
AU - Takahashi, Masayo
AU - Yoshikawa, Kanako
AU - Nishikawa, Shin Ichi
AU - Nakatsuji, Norio
AU - Sasai, Yoshiki
PY - 2002/2/5
Y1 - 2002/2/5
N2 - We previously identified a stromal cell-derived inducing activity (SDIA), which induces differentiation of neural cells, including midbrain tyrosine hydroxylase-positive (TH+) dopaminergic neurons, from mouse embryonic stem cells. We report here that SDIA induces efficient neural differentiation also in primate embryonic stem cells. Induced neurons contain TH+ neurons at a frequency of 35% and produce a significant amount of dopamine. Interestingly, differentiation of TH+ neurons from undifferentiated embryonic cells occurs much faster in vitro (10 days) than it does in the embryo (≈5 weeks). In addition, 8% of the colonies contain large patches of Pax6+-pigmented epithelium of the retina. The SDIA method provides an unlimited source of primate cells for the study of pathogenesis, drug development, and transplantation in degenerative diseases such as Parkinson's disease and retinitis pigmentosa.
AB - We previously identified a stromal cell-derived inducing activity (SDIA), which induces differentiation of neural cells, including midbrain tyrosine hydroxylase-positive (TH+) dopaminergic neurons, from mouse embryonic stem cells. We report here that SDIA induces efficient neural differentiation also in primate embryonic stem cells. Induced neurons contain TH+ neurons at a frequency of 35% and produce a significant amount of dopamine. Interestingly, differentiation of TH+ neurons from undifferentiated embryonic cells occurs much faster in vitro (10 days) than it does in the embryo (≈5 weeks). In addition, 8% of the colonies contain large patches of Pax6+-pigmented epithelium of the retina. The SDIA method provides an unlimited source of primate cells for the study of pathogenesis, drug development, and transplantation in degenerative diseases such as Parkinson's disease and retinitis pigmentosa.
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U2 - 10.1073/pnas.032662199
DO - 10.1073/pnas.032662199
M3 - Article
C2 - 11818560
AN - SCOPUS:0037022299
SN - 0027-8424
VL - 99
SP - 1580
EP - 1585
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 3
ER -