Generation of high affinity monoclonal antibodies for the prevention of HIV infection

Nobuo Sakaguchi, Teppei Toda, Teruo Nakaya, Masataka Kitabatake, Kazuhiko Maeda, Kazuhiko Kuwahara

Research output: Contribution to journalReview articlepeer-review

4 Citations (Scopus)


The most critical issue for the application of high affinity monoclonal antibodies is their creation. Here, we summarize the cellular and molecular mechanisms by which high affinity antibodies are generated, and then review the attempts of many investigators to create high affinity monoclonal antibodies against various target molecules. High affinity monoclonal antibodies are generated by one or a combination of the following three major methods. (1) The improvement of antibody affinity by introducing mutations in the immunoglobulin V-region genes by in vitro mutagenesis. (2) Screening many clones from a random combinatory repertoire of IgV-region genes using a phage library established in yeast or bacteria. (3) Attempting to introduce many somatic hypermutation of IgV-region genes. We summarize the advantages and applications of each of these methods including recent patents to facilitate informed individual choice. We also extend our review to the current creation of antibodies for HIV research.

Original languageEnglish
Pages (from-to)88-95
Number of pages8
JournalRecent Patents on DNA and Gene Sequences
Issue number2
Publication statusPublished - 2009

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Molecular Biology
  • Genetics(clinical)


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