Generation of HLA-A2 subtype specific cytotoxic T lymphocytes from cord blood used for cord blood stem cell transplantation

M. Yazaki, T. Takahashi, Y. Ito, Tetsuya Ito, C. Mori, Y. Wada

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Alloantigen reactive cytotoxic T lymphocytes (CTL) were generated from cord blood (CB) lymphocytes used for cord blood stem cell transplantation (CBSCT). The CTL were cytotoxic against the patient's leukemic cells, as well as the patient's EBV-lymphoblastoid cell line (EBV-LCL), and PHA blasts. The cytotoxicity against patient's EBV-LCL was blocked by anti-HLA-A2 MoAb, and anti-HLA-class I MoAb. The CTL recognized A*0206 positive EBV-LCLs, but not A*0201, A*0204, or A*0207 positive EBV-LCLs, suggesting that this CTL recognizes HLA-A*0206. This case suggests that CB T cells may be competent enough to generate CTL to induce a GVL effect, together with those against A*0206, in patients with CBSCT.

Original languageEnglish
Pages (from-to)451-453
Number of pages3
JournalBone Marrow Transplantation
Volume26
Issue number4
Publication statusPublished - 06-09-2000
Externally publishedYes

Fingerprint

Cord Blood Stem Cell Transplantation
HLA-A2 Antigen
Cytotoxic T-Lymphocytes
Fetal Blood
Human Herpesvirus 4
Cell Line
HLA-A Antigens
Isoantigens
Blood Cells
Lymphocytes
T-Lymphocytes

All Science Journal Classification (ASJC) codes

  • Hematology
  • Transplantation

Cite this

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Generation of HLA-A2 subtype specific cytotoxic T lymphocytes from cord blood used for cord blood stem cell transplantation. / Yazaki, M.; Takahashi, T.; Ito, Y.; Ito, Tetsuya; Mori, C.; Wada, Y.

In: Bone Marrow Transplantation, Vol. 26, No. 4, 06.09.2000, p. 451-453.

Research output: Contribution to journalArticle

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T1 - Generation of HLA-A2 subtype specific cytotoxic T lymphocytes from cord blood used for cord blood stem cell transplantation

AU - Yazaki, M.

AU - Takahashi, T.

AU - Ito, Y.

AU - Ito, Tetsuya

AU - Mori, C.

AU - Wada, Y.

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AB - Alloantigen reactive cytotoxic T lymphocytes (CTL) were generated from cord blood (CB) lymphocytes used for cord blood stem cell transplantation (CBSCT). The CTL were cytotoxic against the patient's leukemic cells, as well as the patient's EBV-lymphoblastoid cell line (EBV-LCL), and PHA blasts. The cytotoxicity against patient's EBV-LCL was blocked by anti-HLA-A2 MoAb, and anti-HLA-class I MoAb. The CTL recognized A*0206 positive EBV-LCLs, but not A*0201, A*0204, or A*0207 positive EBV-LCLs, suggesting that this CTL recognizes HLA-A*0206. This case suggests that CB T cells may be competent enough to generate CTL to induce a GVL effect, together with those against A*0206, in patients with CBSCT.

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