Generation of HLA-C-specific cytotoxic T cells in association with marrow graft rejection: Analysis of alloimmunity by T-cell cloning and testing of T-cell-receptor rearrangements

Ji Pei, Yoshiki Akatsuka, Claudio Anasetti, Ming Tseh Lin, Effie W. Petersdorf, John A. Hansen, Paul J. Martin

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Peripheral blood mononuclear cells from a patient who rejected a T-cell-depleted marrow graft showed spontaneous cytotoxic activity specific for HLA-Cw4 and HLA-B35 alloantigens of the donor. T-cell-receptor complementarity-determining region 3 length distributions in a blood sample obtained at the time of rejection generally showed distortions in only 1 or 2 peaks, indicating that recipient T cells with a broad repertoire of antigen receptors survived the pretransplantation conditioning regimen. An HLA-Cw4-specific, CD8+ T-cell clone was recovered from a blood sample after rejection. The T-cell-receptor-B gene rearrangement expressed by this clone was not readily detectable in other blood samples drawn before or after the transplantation, indicating that this clone was only 1 of many recipient-derived T cells that recognized HLA alloantigens of the donor. These results demonstrate the role of the HLA-C locus in T-cell-mediated alloimmune responses and add to emerging evidence that indicates the need to evaluate HLA-C compatibility in selecting hematopoietic stem cell donors for patients who have an increased risk of rejection.

Original languageEnglish
Pages (from-to)378-383
Number of pages6
JournalBiology of Blood and Marrow Transplantation
Volume7
Issue number7
DOIs
Publication statusPublished - 01-01-2001
Externally publishedYes

Fingerprint

HLA-C Antigens
Graft Rejection
T-Cell Antigen Receptor
Organism Cloning
Bone Marrow
T-Lymphocytes
Isoantigens
Clone Cells
Tissue Donors
HLA-B35 Antigen
Complementarity Determining Regions
T-Cell Receptor Genes
Antigen Receptors
Gene Rearrangement
Hematopoietic Stem Cells
Blood Cells
Transplantation
Transplants

All Science Journal Classification (ASJC) codes

  • Hematology
  • Transplantation

Cite this

Pei, Ji ; Akatsuka, Yoshiki ; Anasetti, Claudio ; Lin, Ming Tseh ; Petersdorf, Effie W. ; Hansen, John A. ; Martin, Paul J. / Generation of HLA-C-specific cytotoxic T cells in association with marrow graft rejection : Analysis of alloimmunity by T-cell cloning and testing of T-cell-receptor rearrangements. In: Biology of Blood and Marrow Transplantation. 2001 ; Vol. 7, No. 7. pp. 378-383.
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Generation of HLA-C-specific cytotoxic T cells in association with marrow graft rejection : Analysis of alloimmunity by T-cell cloning and testing of T-cell-receptor rearrangements. / Pei, Ji; Akatsuka, Yoshiki; Anasetti, Claudio; Lin, Ming Tseh; Petersdorf, Effie W.; Hansen, John A.; Martin, Paul J.

In: Biology of Blood and Marrow Transplantation, Vol. 7, No. 7, 01.01.2001, p. 378-383.

Research output: Contribution to journalArticle

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