Generation of recombinant rotavirus with an antigenic mosaic of cross-reactive neutralization epitopes on VP4

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Recombinant rotavirus (RV) with cDNA-derived chimeric VP4 was generated using recently developed reverse genetics for RV. The rescued virus, KU//rVP4(SA11)-II(DS-1), contains SA11 (simian RV strain, G3P[2])-based VP4, in which a cross-reactive neutralization epitope (amino acids 381 to 401) on VP5* is replaced by the corresponding sequence of a different P-type DS-1 (human RV strain, G2P[4]). Serological analyses with a panel of anti-VP4- and -VP7-neutralizing monoclonal antibodies revealed that the rescued virus carries a novel antigenic mosaic of cross-reactive neutralization epitopes on its VP4 surface. This is the first report of the generation of a recombinant RV with artificial amino acid substitutions.

Original languageEnglish
Pages (from-to)6753-6757
Number of pages5
JournalJournal of Virology
Volume82
Issue number13
DOIs
Publication statusPublished - 01-07-2008

Fingerprint

Rotavirus
neutralization
epitopes
Epitopes
Viruses
Reverse Genetics
viruses
amino acid substitution
Amino Acid Substitution
Neutralizing Antibodies
neutralizing antibodies
monoclonal antibodies
Complementary DNA
Monoclonal Antibodies
Amino Acids
amino acids

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

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abstract = "Recombinant rotavirus (RV) with cDNA-derived chimeric VP4 was generated using recently developed reverse genetics for RV. The rescued virus, KU//rVP4(SA11)-II(DS-1), contains SA11 (simian RV strain, G3P[2])-based VP4, in which a cross-reactive neutralization epitope (amino acids 381 to 401) on VP5* is replaced by the corresponding sequence of a different P-type DS-1 (human RV strain, G2P[4]). Serological analyses with a panel of anti-VP4- and -VP7-neutralizing monoclonal antibodies revealed that the rescued virus carries a novel antigenic mosaic of cross-reactive neutralization epitopes on its VP4 surface. This is the first report of the generation of a recombinant RV with artificial amino acid substitutions.",
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Generation of recombinant rotavirus with an antigenic mosaic of cross-reactive neutralization epitopes on VP4. / Komoto, Satoshi; Kugita, Masanori; Sasaki, Jun; Taniguchi, Koki.

In: Journal of Virology, Vol. 82, No. 13, 01.07.2008, p. 6753-6757.

Research output: Contribution to journalArticle

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