Genes that characterize T3-predominant Graves' thyroid tissues.

Chisa Matsumoto, Mitsuru Ito, Hiroya Yamada, Noriko Yamakawa, Hiroshi Yoshida, Arisa Date, Mikio Watanabe, Yoh Hidaka, Yoshinori Iwatani, Akira Miyauchi, Toru Takano

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

3,5,3'-Triiodothyronine (T(3))-predominant Graves' disease is characterized by the increasing volume of thyroid goiter resulting in poor prognosis. Although type 1 and type 2 iodothyronine deiodinases (DIO1 and DIO2 respectively) are known to be overexpressed in the thyroid tissues of T(3)-predominant Graves' disease, the pathogenesis of this disease is still unclear. The aim of our study is to identify genes that characterize T(3)-predominant Graves' disease tissue in order to clarify the molecular mechanism of this disease. mRNAs from two thyroid tissues of both typical T(3)-predominant and common-type Graves' disease were analyzed with DNA microarrays with probes for 28 869 genes. Genes identified to be differentially expressed between the two groups were further analyzed in the second and third screenings using 70 Graves' thyroid tissues by real-time quantitative RT-PCR. Twenty-three candidate genes were selected as being differentially expressed in the first screening with microarrays. Among these, seven genes, leucine-rich repeat neuronal 1 (LRRN1), bone morphogenetic protein 8a (BMP8A), N-cadherin (CDH2), phosphodiesterase 1A (PDE1A), creatine kinase mitochondrial 2 (CKMT2), integrin beta-3 (ITGB3), and protein tyrosine phosphatase non-receptor type 4 (PTPN4), were confirmed to be differentially expressed in DIO1 or DIO2 over- and underexpressing Graves' tissues. These genes are related to the characteristics of T(3)-predominant Graves' disease, such as high titer level of serum anti-TSH receptor antibody, high free T(3) to free thyroxine ratio, and a large goiter size. They might play a role in the pathogenesis of T(3)-predominant Graves' disease.

Original languageEnglish
Pages (from-to)137-144
Number of pages8
JournalUnknown Journal
Volume168
Issue number2
DOIs
Publication statusPublished - 01-01-2013

Fingerprint

Graves Disease
Thyroid Gland
Genes
Non-Receptor Type 4 Protein Tyrosine Phosphatase
Goiter
Non-Receptor Type 3 Protein Tyrosine Phosphatase
Bone Morphogenetic Protein 1
Mitochondrial Form Creatine Kinase
Integrin beta Chains
Iodide Peroxidase
Phosphoric Diester Hydrolases
Triiodothyronine
Cadherins
Oligonucleotide Array Sequence Analysis
Thyroxine
Leucine
Real-Time Polymerase Chain Reaction
Messenger RNA
Serum

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Matsumoto, C., Ito, M., Yamada, H., Yamakawa, N., Yoshida, H., Date, A., ... Takano, T. (2013). Genes that characterize T3-predominant Graves' thyroid tissues. Unknown Journal, 168(2), 137-144. https://doi.org/10.1530/EJE-12-0507
Matsumoto, Chisa ; Ito, Mitsuru ; Yamada, Hiroya ; Yamakawa, Noriko ; Yoshida, Hiroshi ; Date, Arisa ; Watanabe, Mikio ; Hidaka, Yoh ; Iwatani, Yoshinori ; Miyauchi, Akira ; Takano, Toru. / Genes that characterize T3-predominant Graves' thyroid tissues. In: Unknown Journal. 2013 ; Vol. 168, No. 2. pp. 137-144.
@article{6825f41f3893441c815e936a39003d38,
title = "Genes that characterize T3-predominant Graves' thyroid tissues.",
abstract = "3,5,3'-Triiodothyronine (T(3))-predominant Graves' disease is characterized by the increasing volume of thyroid goiter resulting in poor prognosis. Although type 1 and type 2 iodothyronine deiodinases (DIO1 and DIO2 respectively) are known to be overexpressed in the thyroid tissues of T(3)-predominant Graves' disease, the pathogenesis of this disease is still unclear. The aim of our study is to identify genes that characterize T(3)-predominant Graves' disease tissue in order to clarify the molecular mechanism of this disease. mRNAs from two thyroid tissues of both typical T(3)-predominant and common-type Graves' disease were analyzed with DNA microarrays with probes for 28 869 genes. Genes identified to be differentially expressed between the two groups were further analyzed in the second and third screenings using 70 Graves' thyroid tissues by real-time quantitative RT-PCR. Twenty-three candidate genes were selected as being differentially expressed in the first screening with microarrays. Among these, seven genes, leucine-rich repeat neuronal 1 (LRRN1), bone morphogenetic protein 8a (BMP8A), N-cadherin (CDH2), phosphodiesterase 1A (PDE1A), creatine kinase mitochondrial 2 (CKMT2), integrin beta-3 (ITGB3), and protein tyrosine phosphatase non-receptor type 4 (PTPN4), were confirmed to be differentially expressed in DIO1 or DIO2 over- and underexpressing Graves' tissues. These genes are related to the characteristics of T(3)-predominant Graves' disease, such as high titer level of serum anti-TSH receptor antibody, high free T(3) to free thyroxine ratio, and a large goiter size. They might play a role in the pathogenesis of T(3)-predominant Graves' disease.",
author = "Chisa Matsumoto and Mitsuru Ito and Hiroya Yamada and Noriko Yamakawa and Hiroshi Yoshida and Arisa Date and Mikio Watanabe and Yoh Hidaka and Yoshinori Iwatani and Akira Miyauchi and Toru Takano",
year = "2013",
month = "1",
day = "1",
doi = "10.1530/EJE-12-0507",
language = "English",
volume = "168",
pages = "137--144",
journal = "Unknown Journal",
number = "2",

}

Matsumoto, C, Ito, M, Yamada, H, Yamakawa, N, Yoshida, H, Date, A, Watanabe, M, Hidaka, Y, Iwatani, Y, Miyauchi, A & Takano, T 2013, 'Genes that characterize T3-predominant Graves' thyroid tissues.', Unknown Journal, vol. 168, no. 2, pp. 137-144. https://doi.org/10.1530/EJE-12-0507

Genes that characterize T3-predominant Graves' thyroid tissues. / Matsumoto, Chisa; Ito, Mitsuru; Yamada, Hiroya; Yamakawa, Noriko; Yoshida, Hiroshi; Date, Arisa; Watanabe, Mikio; Hidaka, Yoh; Iwatani, Yoshinori; Miyauchi, Akira; Takano, Toru.

In: Unknown Journal, Vol. 168, No. 2, 01.01.2013, p. 137-144.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Genes that characterize T3-predominant Graves' thyroid tissues.

AU - Matsumoto, Chisa

AU - Ito, Mitsuru

AU - Yamada, Hiroya

AU - Yamakawa, Noriko

AU - Yoshida, Hiroshi

AU - Date, Arisa

AU - Watanabe, Mikio

AU - Hidaka, Yoh

AU - Iwatani, Yoshinori

AU - Miyauchi, Akira

AU - Takano, Toru

PY - 2013/1/1

Y1 - 2013/1/1

N2 - 3,5,3'-Triiodothyronine (T(3))-predominant Graves' disease is characterized by the increasing volume of thyroid goiter resulting in poor prognosis. Although type 1 and type 2 iodothyronine deiodinases (DIO1 and DIO2 respectively) are known to be overexpressed in the thyroid tissues of T(3)-predominant Graves' disease, the pathogenesis of this disease is still unclear. The aim of our study is to identify genes that characterize T(3)-predominant Graves' disease tissue in order to clarify the molecular mechanism of this disease. mRNAs from two thyroid tissues of both typical T(3)-predominant and common-type Graves' disease were analyzed with DNA microarrays with probes for 28 869 genes. Genes identified to be differentially expressed between the two groups were further analyzed in the second and third screenings using 70 Graves' thyroid tissues by real-time quantitative RT-PCR. Twenty-three candidate genes were selected as being differentially expressed in the first screening with microarrays. Among these, seven genes, leucine-rich repeat neuronal 1 (LRRN1), bone morphogenetic protein 8a (BMP8A), N-cadherin (CDH2), phosphodiesterase 1A (PDE1A), creatine kinase mitochondrial 2 (CKMT2), integrin beta-3 (ITGB3), and protein tyrosine phosphatase non-receptor type 4 (PTPN4), were confirmed to be differentially expressed in DIO1 or DIO2 over- and underexpressing Graves' tissues. These genes are related to the characteristics of T(3)-predominant Graves' disease, such as high titer level of serum anti-TSH receptor antibody, high free T(3) to free thyroxine ratio, and a large goiter size. They might play a role in the pathogenesis of T(3)-predominant Graves' disease.

AB - 3,5,3'-Triiodothyronine (T(3))-predominant Graves' disease is characterized by the increasing volume of thyroid goiter resulting in poor prognosis. Although type 1 and type 2 iodothyronine deiodinases (DIO1 and DIO2 respectively) are known to be overexpressed in the thyroid tissues of T(3)-predominant Graves' disease, the pathogenesis of this disease is still unclear. The aim of our study is to identify genes that characterize T(3)-predominant Graves' disease tissue in order to clarify the molecular mechanism of this disease. mRNAs from two thyroid tissues of both typical T(3)-predominant and common-type Graves' disease were analyzed with DNA microarrays with probes for 28 869 genes. Genes identified to be differentially expressed between the two groups were further analyzed in the second and third screenings using 70 Graves' thyroid tissues by real-time quantitative RT-PCR. Twenty-three candidate genes were selected as being differentially expressed in the first screening with microarrays. Among these, seven genes, leucine-rich repeat neuronal 1 (LRRN1), bone morphogenetic protein 8a (BMP8A), N-cadherin (CDH2), phosphodiesterase 1A (PDE1A), creatine kinase mitochondrial 2 (CKMT2), integrin beta-3 (ITGB3), and protein tyrosine phosphatase non-receptor type 4 (PTPN4), were confirmed to be differentially expressed in DIO1 or DIO2 over- and underexpressing Graves' tissues. These genes are related to the characteristics of T(3)-predominant Graves' disease, such as high titer level of serum anti-TSH receptor antibody, high free T(3) to free thyroxine ratio, and a large goiter size. They might play a role in the pathogenesis of T(3)-predominant Graves' disease.

UR - http://www.scopus.com/inward/record.url?scp=84874537292&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84874537292&partnerID=8YFLogxK

U2 - 10.1530/EJE-12-0507

DO - 10.1530/EJE-12-0507

M3 - Article

C2 - 23109646

AN - SCOPUS:84874537292

VL - 168

SP - 137

EP - 144

JO - Unknown Journal

JF - Unknown Journal

IS - 2

ER -

Matsumoto C, Ito M, Yamada H, Yamakawa N, Yoshida H, Date A et al. Genes that characterize T3-predominant Graves' thyroid tissues. Unknown Journal. 2013 Jan 1;168(2):137-144. https://doi.org/10.1530/EJE-12-0507