Genetic engineering of rotaviruses by reverse genetics

Satoshi Komoto, Koki Taniguchi

Research output: Contribution to journalReview article

9 Citations (Scopus)

Abstract

The rotavirus genome is composed of 11 gene segments of dsRNA. A recent breakthrough in the field of rotaviruses is the development of a reverse genetics system for generating recombinant rotaviruses possessing a gene segment derived from cloned cDNA. Although this approach is a helper virus-driven system that is technically limited and gives low levels of recombinant viruses, it allows alteration of the rotavirus genome, thus contributing to our understanding of these medically important viruses. So far, this approach has successfully been applied to three of the 11 viral segments in our laboratory and others, and the efficiency of recovery of recombinant viruses has been improved. However, we are still waiting for the development of a helper virus-free reverse genetics system for generating an infectious rotavirus entirely from cDNAs, as has been achieved for other members of the Reoviridae family.

Original languageEnglish
Pages (from-to)479-486
Number of pages8
JournalMICROBIOLOGY and IMMUNOLOGY
Volume57
Issue number7
DOIs
Publication statusPublished - 01-07-2013

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

Cite this