TY - JOUR
T1 - Genetic Variation in the Indoleamine 2,3-Dioxygenase Gene in Pre-eclampsia
AU - Nishizawa, Haruki
AU - Kato, Takema
AU - Ota, Sayuri
AU - Nishiyama, Sachie
AU - Pryor-Koishi, Kanako
AU - Suzuki, Machiko
AU - Tsutsumi, Makiko
AU - Inagaki, Hidehito
AU - Kurahashi, Hiroki
AU - Udagawa, Yasuhiro
PY - 2010/7
Y1 - 2010/7
N2 - Problem To investigate the contribution of genomic variations in the indoleamine 2,3-dioxygenase (IDO) gene to the onset of pre-eclampsia.Method of study We examined sequence variations in the IDO1 gene using placental genomic DNA from 35 pre-eclamptic patients and 32 normotensive pregnant women.Results A case-control study revealed that none of the common variants influences the risk of disease. Sequencing of each IDO1 exon in diseased subjects revealed rare variants. This variation, c.-147_150delGAAA, was located within the 5'-untranslated region of the IDO1 gene, and its homozygote was identified only in pre-eclamptic subjects. However, despite the low levels of IDO expression and enzyme activity in the c.-147_150delGAAA homozygote, reporter assays indicated that this variation does not affect gene expression.Conclusion Our findings indicate that genetic alteration of fetal IDO gene does not appear to be a primary cause of pre-eclampsia.
AB - Problem To investigate the contribution of genomic variations in the indoleamine 2,3-dioxygenase (IDO) gene to the onset of pre-eclampsia.Method of study We examined sequence variations in the IDO1 gene using placental genomic DNA from 35 pre-eclamptic patients and 32 normotensive pregnant women.Results A case-control study revealed that none of the common variants influences the risk of disease. Sequencing of each IDO1 exon in diseased subjects revealed rare variants. This variation, c.-147_150delGAAA, was located within the 5'-untranslated region of the IDO1 gene, and its homozygote was identified only in pre-eclamptic subjects. However, despite the low levels of IDO expression and enzyme activity in the c.-147_150delGAAA homozygote, reporter assays indicated that this variation does not affect gene expression.Conclusion Our findings indicate that genetic alteration of fetal IDO gene does not appear to be a primary cause of pre-eclampsia.
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U2 - 10.1111/j.1600-0897.2010.00820.x
DO - 10.1111/j.1600-0897.2010.00820.x
M3 - Article
C2 - 20192952
AN - SCOPUS:77955973362
SN - 1046-7408
VL - 64
SP - 68
EP - 76
JO - American Journal of Reproductive Immunology
JF - American Journal of Reproductive Immunology
IS - 1
ER -