Genetic Variation in the Indoleamine 2,3-Dioxygenase Gene in Pre-eclampsia

Haruki Nishizawa, Takema Kato, Sayuri Ota, Sachie Nishiyama, Kanako Pryor-Koishi, Machiko Suzuki, Makiko Tsutsumi, Hidehito Inagaki, Hiroki Kurahashi, Yasuhiro Udagawa

Research output: Contribution to journalArticle

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Abstract

Problem To investigate the contribution of genomic variations in the indoleamine 2,3-dioxygenase (IDO) gene to the onset of pre-eclampsia.Method of study We examined sequence variations in the IDO1 gene using placental genomic DNA from 35 pre-eclamptic patients and 32 normotensive pregnant women.Results A case-control study revealed that none of the common variants influences the risk of disease. Sequencing of each IDO1 exon in diseased subjects revealed rare variants. This variation, c.-147_150delGAAA, was located within the 5'-untranslated region of the IDO1 gene, and its homozygote was identified only in pre-eclamptic subjects. However, despite the low levels of IDO expression and enzyme activity in the c.-147_150delGAAA homozygote, reporter assays indicated that this variation does not affect gene expression.Conclusion Our findings indicate that genetic alteration of fetal IDO gene does not appear to be a primary cause of pre-eclampsia.

Original languageEnglish
Pages (from-to)68-76
Number of pages9
JournalAmerican Journal of Reproductive Immunology
Volume64
Issue number1
DOIs
Publication statusPublished - 01-07-2010

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Indoleamine-Pyrrole 2,3,-Dioxygenase
Pre-Eclampsia
Homozygote
Genes
5' Untranslated Regions
Case-Control Studies
Pregnant Women
Exons
Gene Expression
DNA
Enzymes

All Science Journal Classification (ASJC) codes

  • Immunology
  • Immunology and Allergy
  • Obstetrics and Gynaecology
  • Reproductive Medicine
  • Medicine(all)

Cite this

Nishizawa, Haruki ; Kato, Takema ; Ota, Sayuri ; Nishiyama, Sachie ; Pryor-Koishi, Kanako ; Suzuki, Machiko ; Tsutsumi, Makiko ; Inagaki, Hidehito ; Kurahashi, Hiroki ; Udagawa, Yasuhiro. / Genetic Variation in the Indoleamine 2,3-Dioxygenase Gene in Pre-eclampsia. In: American Journal of Reproductive Immunology. 2010 ; Vol. 64, No. 1. pp. 68-76.
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Genetic Variation in the Indoleamine 2,3-Dioxygenase Gene in Pre-eclampsia. / Nishizawa, Haruki; Kato, Takema; Ota, Sayuri; Nishiyama, Sachie; Pryor-Koishi, Kanako; Suzuki, Machiko; Tsutsumi, Makiko; Inagaki, Hidehito; Kurahashi, Hiroki; Udagawa, Yasuhiro.

In: American Journal of Reproductive Immunology, Vol. 64, No. 1, 01.07.2010, p. 68-76.

Research output: Contribution to journalArticle

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AU - Suzuki, Machiko

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N2 - Problem To investigate the contribution of genomic variations in the indoleamine 2,3-dioxygenase (IDO) gene to the onset of pre-eclampsia.Method of study We examined sequence variations in the IDO1 gene using placental genomic DNA from 35 pre-eclamptic patients and 32 normotensive pregnant women.Results A case-control study revealed that none of the common variants influences the risk of disease. Sequencing of each IDO1 exon in diseased subjects revealed rare variants. This variation, c.-147_150delGAAA, was located within the 5'-untranslated region of the IDO1 gene, and its homozygote was identified only in pre-eclamptic subjects. However, despite the low levels of IDO expression and enzyme activity in the c.-147_150delGAAA homozygote, reporter assays indicated that this variation does not affect gene expression.Conclusion Our findings indicate that genetic alteration of fetal IDO gene does not appear to be a primary cause of pre-eclampsia.

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