Genistein inhibits Ca2+ influx by extracellular ATP in PC12 pheochromocytoma cells

O. Kozawa, J. Shinoda, Atsushi Suzuki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We previously showed that extracellular ATP-induced Ca2+ influx is inhibited by self-activated protein kinase C in rat PC12 pheochromocytoma cells. In the present study, we examined whether tyrosine kinase is involved in the ATP-induced Ca2+ influx in PC12 cells. Genistein, an inhibitor of protein tyrosine kinases, which by itself had little effect on 45Ca2+ influx, significantly suppressed the ATP-induced 45Ca2+ influx in a dose-dependent manner in the range between 1 and 30 μg/ml. Tyrphostin, another inhibitor of tyrosine kinases chemically distinct from genistein, also inhibited the 45Ca2+ influx. Sodium orthovanadate, an inhibitor of protein tyrosine phosphatases, markedly enhanced the ATP-induced 45Ca2+ influx. These results suggest that tyrosine kinase regulates Ca2+ influx induced by extracellular ATP in PC12 pheochromocytoma cells.

Original languageEnglish
Pages (from-to)272-274
Number of pages3
JournalHormone and Metabolic Research
Volume27
Issue number6
Publication statusPublished - 01-01-1995
Externally publishedYes

Fingerprint

Genistein
PC12 Cells
Pheochromocytoma
Adenosine Triphosphate
Protein-Tyrosine Kinases
Tyrphostins
Protein Tyrosine Phosphatases
Vanadates
Protein Kinase C
Rats
Sodium

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

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abstract = "We previously showed that extracellular ATP-induced Ca2+ influx is inhibited by self-activated protein kinase C in rat PC12 pheochromocytoma cells. In the present study, we examined whether tyrosine kinase is involved in the ATP-induced Ca2+ influx in PC12 cells. Genistein, an inhibitor of protein tyrosine kinases, which by itself had little effect on 45Ca2+ influx, significantly suppressed the ATP-induced 45Ca2+ influx in a dose-dependent manner in the range between 1 and 30 μg/ml. Tyrphostin, another inhibitor of tyrosine kinases chemically distinct from genistein, also inhibited the 45Ca2+ influx. Sodium orthovanadate, an inhibitor of protein tyrosine phosphatases, markedly enhanced the ATP-induced 45Ca2+ influx. These results suggest that tyrosine kinase regulates Ca2+ influx induced by extracellular ATP in PC12 pheochromocytoma cells.",
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Genistein inhibits Ca2+ influx by extracellular ATP in PC12 pheochromocytoma cells. / Kozawa, O.; Shinoda, J.; Suzuki, Atsushi.

In: Hormone and Metabolic Research, Vol. 27, No. 6, 01.01.1995, p. 272-274.

Research output: Contribution to journalArticle

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