Genome-wide analysis reveals phytohormone action during cassava storage root initiation

Punchapat Sojikul, Treenut Saithong, Saowalak Kalapanulak, Nuttapat Pisuttinusart, Siripan Limsirichaikul, Maho Tanaka, Yoshinori Utsumi, Tetsuya Sakurai, Motoaki Seki, Jarunya Narangajavana

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

Development of storage roots is a process associated with a phase change from cell division and elongation to radial growth and accumulation of massive amounts of reserve substances such as starch. Knowledge of the regulation of cassava storage root formation has accumulated over time; however, gene regulation during the initiation and early stage of storage root development is still poorly understood. In this study, transcription profiling of fibrous, intermediate and storage roots at eight weeks old were investigated using a 60-mer-oligo microarray. Transcription and gene expression were found to be the key regulating processes during the transition stage from fibrous to intermediate roots, while homeostasis and signal transduction influenced regulation from intermediate roots to storage roots. Clustering analysis of significant genes and transcription factors (TF) indicated that a number of phytohormone-related TF were differentially expressed; therefore, phytohormone-related genes were assembled into a network of correlative nodes. We propose a model showing the relationship between KNOX1 and phytohormones during storage root initiation. Exogeneous treatment of phytohormones N6-benzylaminopurine and 1-Naphthaleneacetic acid were used to induce the storage root initiation stage and to investigate expression patterns of the genes involved in storage root initiation. The results support the hypothesis that phytohormones are acting in concert to regulate the onset of cassava storage root development. Moreover, MeAGL20 is a factor that might play an important role at the onset of storage root initiation when the root tip becomes swollen.

Original languageEnglish
Pages (from-to)531-543
Number of pages13
JournalPlant Molecular Biology
Volume88
Issue number6
DOIs
Publication statusPublished - 05-08-2015
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Fingerprint

Dive into the research topics of 'Genome-wide analysis reveals phytohormone action during cassava storage root initiation'. Together they form a unique fingerprint.

Cite this