TY - JOUR
T1 - Genomic analysis of group A rotavirus G12P[8] including a new Japanese strain revealed evidence for intergenotypic recombination in VP7 and VP4 genes
AU - Phan, Tung
AU - Ide, Tomihiko
AU - Komoto, Satoshi
AU - Khamrin, Pattara
AU - Pham, Ngan Thi Kim
AU - Okitsu, Shoko
AU - Taniguchi, Koki
AU - Nishimura, Shuichi
AU - Maneekarn, Niwat
AU - Hayakawa, Satoshi
AU - Ushijima, Hiroshi
N1 - Funding Information:
We thank Mrs. Itoe Shiota, Mrs. Yuko Onda-Shimizu at Nihon University, School of Medicine for helping laboratory experiments, and Dr. Xianfeng Chen at Department of Health Sciences Research & Center for Individualized Medicine, Mayo Clinic, Scottsdale, AZ, USA for analyzing the data. This work was supported by Grant-in-Aid for Scientific Research ( 16H05360 ) and Public Foundation of the Vaccination Research Center ( 2018-38 , 2019-41 ).
Funding Information:
We thank Mrs. Itoe Shiota, Mrs. Yuko Onda-Shimizu at Nihon University, School of Medicine for helping laboratory experiments, and Dr. Xianfeng Chen at Department of Health Sciences Research & Center for Individualized Medicine, Mayo Clinic, Scottsdale, AZ, USA for analyzing the data. This work was supported by Grant-in-Aid for Scientific Research (16H05360) and Public Foundation of the Vaccination Research Center (2018-38, 2019-41).
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2021/1
Y1 - 2021/1
N2 - Group A rotavirus is a leading cause of severe acute gastroenteritis worldwide. In this study, the first complete coding sequences of 11 RNA segments of human group A rotavirus G12P[8] in Japan were determined by an unbiased viral metagenomics. Its genomic constellation (VP7-VP4-VP6-VP1-VP2-VP3-NSP1-NSP2-NSP3-NSP4-NSP5 genes) was identified as G12-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1. When performing the genetic analysis, we discovered an intergenotypic recombination event in the pig group A rotavirus G12P[8] strain BUW-14-A008. The novel recombination was found between two different genotypes G12 and G3 in the VP7 gene, and P[8] and P[13] in the VP4 gene.
AB - Group A rotavirus is a leading cause of severe acute gastroenteritis worldwide. In this study, the first complete coding sequences of 11 RNA segments of human group A rotavirus G12P[8] in Japan were determined by an unbiased viral metagenomics. Its genomic constellation (VP7-VP4-VP6-VP1-VP2-VP3-NSP1-NSP2-NSP3-NSP4-NSP5 genes) was identified as G12-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1. When performing the genetic analysis, we discovered an intergenotypic recombination event in the pig group A rotavirus G12P[8] strain BUW-14-A008. The novel recombination was found between two different genotypes G12 and G3 in the VP7 gene, and P[8] and P[13] in the VP4 gene.
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U2 - 10.1016/j.meegid.2020.104656
DO - 10.1016/j.meegid.2020.104656
M3 - Article
C2 - 33278636
AN - SCOPUS:85097467714
SN - 1567-1348
VL - 87
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
M1 - 104656
ER -