Genomic characterization of uncommon human G3P[6] rotavirus strains that have emerged in Kenya after rotavirus vaccine introduction, and pre-vaccine human G8P[4] rotavirus strains

Ernest Apondi Wandera, Satoshi Komoto, Shah Mohammad, Tomihiko Ide, Martin Bundi, James Nyangao, Cyrus Kathiiko, Erick Odoyo, Amina Galata, Gabriel Miring'u, Saori Fukuda, Riona Hatazawa, Takayuki Murata, Koki Taniguchi, Yoshio Ichinose

Research output: Contribution to journalArticle

Abstract

A monovalent rotavirus vaccine (RV1) was introduced to the national immunization program in Kenya in July 2014. There was increased detection of uncommon G3P[6] strains that coincided temporally with the timing of this vaccine introduction. Here, we sequenced and characterized the full genomes of two post-vaccine G3P[6] strains, RVA/Human-wt/KEN/KDH1951/2014/G3P[6] and RVA/Human-wt/KEN/KDH1968/2014/G3P[6], as representatives of these uncommon strains. On full-genomic analysis, both strains exhibited a DS-1-like genotype constellation: G3-P[6]-I2-R2-C2-M2-A2-N2-T2-E2-H2. Phylogenetic analysis revealed that all 11 genes of strains KDH1951 and KDH1968 were very closely related to those of human G3P[6] strains isolated in Uganda in 2012–2013, indicating the derivation of these G3P[6] strains from a common ancestor. Because the uncommon G3P[6] strains that emerged in Kenya are fully heterotypic as to the introduced vaccine strain regarding the genotype constellation, vaccine effectiveness against these G3P[6] strains needs to be closely monitored.

Original languageEnglish
Pages (from-to)231-248
Number of pages18
JournalInfection, Genetics and Evolution
Volume68
DOIs
Publication statusPublished - 01-03-2019

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Rotavirus Vaccines
Rotavirus
Kenya
vaccine
genomics
Vaccines
vaccines
Genotype
genotype
Immunization Programs
Uganda
strain analysis
varespladib methyl
immunization
common ancestry
Genome
genome
phylogenetics
gene
Genes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Wandera, Ernest Apondi ; Komoto, Satoshi ; Mohammad, Shah ; Ide, Tomihiko ; Bundi, Martin ; Nyangao, James ; Kathiiko, Cyrus ; Odoyo, Erick ; Galata, Amina ; Miring'u, Gabriel ; Fukuda, Saori ; Hatazawa, Riona ; Murata, Takayuki ; Taniguchi, Koki ; Ichinose, Yoshio. / Genomic characterization of uncommon human G3P[6] rotavirus strains that have emerged in Kenya after rotavirus vaccine introduction, and pre-vaccine human G8P[4] rotavirus strains. In: Infection, Genetics and Evolution. 2019 ; Vol. 68. pp. 231-248.
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abstract = "A monovalent rotavirus vaccine (RV1) was introduced to the national immunization program in Kenya in July 2014. There was increased detection of uncommon G3P[6] strains that coincided temporally with the timing of this vaccine introduction. Here, we sequenced and characterized the full genomes of two post-vaccine G3P[6] strains, RVA/Human-wt/KEN/KDH1951/2014/G3P[6] and RVA/Human-wt/KEN/KDH1968/2014/G3P[6], as representatives of these uncommon strains. On full-genomic analysis, both strains exhibited a DS-1-like genotype constellation: G3-P[6]-I2-R2-C2-M2-A2-N2-T2-E2-H2. Phylogenetic analysis revealed that all 11 genes of strains KDH1951 and KDH1968 were very closely related to those of human G3P[6] strains isolated in Uganda in 2012–2013, indicating the derivation of these G3P[6] strains from a common ancestor. Because the uncommon G3P[6] strains that emerged in Kenya are fully heterotypic as to the introduced vaccine strain regarding the genotype constellation, vaccine effectiveness against these G3P[6] strains needs to be closely monitored.",
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Wandera, EA, Komoto, S, Mohammad, S, Ide, T, Bundi, M, Nyangao, J, Kathiiko, C, Odoyo, E, Galata, A, Miring'u, G, Fukuda, S, Hatazawa, R, Murata, T, Taniguchi, K & Ichinose, Y 2019, 'Genomic characterization of uncommon human G3P[6] rotavirus strains that have emerged in Kenya after rotavirus vaccine introduction, and pre-vaccine human G8P[4] rotavirus strains', Infection, Genetics and Evolution, vol. 68, pp. 231-248. https://doi.org/10.1016/j.meegid.2018.12.004

Genomic characterization of uncommon human G3P[6] rotavirus strains that have emerged in Kenya after rotavirus vaccine introduction, and pre-vaccine human G8P[4] rotavirus strains. / Wandera, Ernest Apondi; Komoto, Satoshi; Mohammad, Shah; Ide, Tomihiko; Bundi, Martin; Nyangao, James; Kathiiko, Cyrus; Odoyo, Erick; Galata, Amina; Miring'u, Gabriel; Fukuda, Saori; Hatazawa, Riona; Murata, Takayuki; Taniguchi, Koki; Ichinose, Yoshio.

In: Infection, Genetics and Evolution, Vol. 68, 01.03.2019, p. 231-248.

Research output: Contribution to journalArticle

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AU - Komoto, Satoshi

AU - Mohammad, Shah

AU - Ide, Tomihiko

AU - Bundi, Martin

AU - Nyangao, James

AU - Kathiiko, Cyrus

AU - Odoyo, Erick

AU - Galata, Amina

AU - Miring'u, Gabriel

AU - Fukuda, Saori

AU - Hatazawa, Riona

AU - Murata, Takayuki

AU - Taniguchi, Koki

AU - Ichinose, Yoshio

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