TY - JOUR
T1 - Genotype of GB virus C/hepatitis G virus by molecular evolutionary analysis
AU - Kondo, Yutaka
AU - Mizokami, Masashi
AU - Nakano, Tatsunori
AU - Kato, Takanobu
AU - Ohba, Ken Ichi
AU - Orito, Etsuro
AU - Ueda, Ryuzo
AU - Mukaide, Motokazu
AU - Hikiji, Kazumasa
AU - Oyunsuren, Tsendsuren
AU - Cooksley, W. Graham
N1 - Funding Information:
Dr M. Mizokami was supported in part by a Grant-in-Aid for Scientific Research (06670571) from the Japanese Ministry of Education, Science and Culture, by agent from the Japanese Ministry of Health and Welfare, Health Science Research Grants (Non-A, non-B Hepatitis Research Grants), from the Japanese International Cooperation Agency, and by the Viral Hepatitis Research Foundation of Japan.
PY - 1997/12
Y1 - 1997/12
N2 - GB virus C/hepatitis G virus is a newly described virus. Classification of GB virus C/hepatitis G virus into genotypes has not been established. We analyzed nucleotide sequences within the 5' untranslated region of GB virus C/hepatitis G virus isolates and segregated these isolates into genotypes. Twenty serum samples with GB virus C/hepatitis G virus RNA from Australia, Cameroon, the Congo, Japan, Mongolia, and Bangladesh were studied. Reverse transcription and polymerase chain reaction were used to obtain GB virus C/hepatitis G virus RNA. After nucleotide sequences from the 5' untranslated region were determined, 68 nucleotide sequences, including 48 previously reported sequences, were analyzed by molecular evolutionary methods. The phylogenetic tree of the 5' untranslated region showed that all strains could be divided into three major genotypes, GB type (type 1), HG type (type 2), and Asian type (type 3). Bootstrap analysis indicated that the strains could be divided into three major Genotypes but could not be further subdivided. Moreover, frequency histograms of pairwise distances between nucleotide sequences demonstrated only one peak. These result indicated that GB virus C/hepatitis G virus can be classified into three major genotypes, GB type (type 1), HG type (type 2), and Asian type (type 3), and should not be divided into minor subtypes.
AB - GB virus C/hepatitis G virus is a newly described virus. Classification of GB virus C/hepatitis G virus into genotypes has not been established. We analyzed nucleotide sequences within the 5' untranslated region of GB virus C/hepatitis G virus isolates and segregated these isolates into genotypes. Twenty serum samples with GB virus C/hepatitis G virus RNA from Australia, Cameroon, the Congo, Japan, Mongolia, and Bangladesh were studied. Reverse transcription and polymerase chain reaction were used to obtain GB virus C/hepatitis G virus RNA. After nucleotide sequences from the 5' untranslated region were determined, 68 nucleotide sequences, including 48 previously reported sequences, were analyzed by molecular evolutionary methods. The phylogenetic tree of the 5' untranslated region showed that all strains could be divided into three major genotypes, GB type (type 1), HG type (type 2), and Asian type (type 3). Bootstrap analysis indicated that the strains could be divided into three major Genotypes but could not be further subdivided. Moreover, frequency histograms of pairwise distances between nucleotide sequences demonstrated only one peak. These result indicated that GB virus C/hepatitis G virus can be classified into three major genotypes, GB type (type 1), HG type (type 2), and Asian type (type 3), and should not be divided into minor subtypes.
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U2 - 10.1016/S0168-1702(97)00123-8
DO - 10.1016/S0168-1702(97)00123-8
M3 - Article
C2 - 9495537
AN - SCOPUS:0031453921
SN - 0168-1702
VL - 52
SP - 221
EP - 230
JO - Virus Research
JF - Virus Research
IS - 2
ER -
