Germ cell survival through carbohydrate-mediated interaction with Sertoli cells

Tomoya O. Akama, Hiroaki Nakagawa, Kazuhiro Sugihara, Sonoko Narisawa, Chikara Ohyama, Shin Ichiro Nishimura, Deborah A. O'Brien, Kelley W. Moremen, José Luis Millán, Michiko N. Fukuda

Research output: Contribution to journalArticlepeer-review

119 Citations (Scopus)

Abstract

Spermatogenesis is a precisely regulated process in which the germ cells closely interact with Sertoli cells. The molecular basis of this cell-cell adhesion is unknown. Here, we demonstrate that targeted disruption of Man2a2, a gene encoding α-mannosidase llx (MX), an enzyme that forms intermediate asparagine-linked carbohydrates (N-glycans), results in Man2a2 null males that are largely infertile. The Man2a2 null spermatogenic cells fail to adhere to Sertoli cells and are prematurely released from the testis to epididymis. We identified an N-glycan structure that plays a key role in germ cell-Sertoli cell adhesion and showed that a specific carbohydrate was required for spermatogenesis.

Original languageEnglish
Pages (from-to)124-127
Number of pages4
JournalScience
Volume295
Issue number5552
DOIs
Publication statusPublished - 04-01-2002

All Science Journal Classification (ASJC) codes

  • General

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