Girdin locates in centrosome and midbody and plays an important role in cell division

Jing Zhuo Mao, Ping Jiang, Su Ping Cui, Ya Li Ren, Jing Zhao, Xiu Hua Yin, Atsushi Enomoto, Hai Jing Liu, Lin Hou, Masahide Takahashi, Bo Zhang

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Girdin is a downstream effector of epidermal growth factor receptor (EGFR)-AKT and interacts with actin and microtubule. Increasing evidence confirmed that Girdin played an important role in cell migration. Here we report that Girdin also regulates cell division. Overexpression or suppression of Girdin leads to attenuated cell proliferation. Imaging of mitotic cells revealed that Girdin is located in the cell division apparatus such as centrosome and midbody. The sub-cellular localization of Girdin was dependent on the domains, which interacted with actin or microtubules. Overexpression of Girdin lead to increased centrosome splitting and amplification. In addition, data show that pAKT also locates in both the centrosome and midbody, indicating the regulating role of AKT in Girdin-mediated cell division. To elucidate the effect of Girdin on tumor growth in vivo, HeLa cells infected with retrovirus harboring either control or Girdin shRNAs were injected subcutaneously into the immunocompromised nude mice. Downregulation of Girdin by shRNA markedly inhibited the cell growth of subcutaneously transplanted tumors in nude mice. These data demonstrate that Girdin is important for efficient cell division. Taking our previous data into consideration, we speculate that Girdin regulates both cell division and cell migration through cytoskeletal molecules.

Original languageEnglish
Pages (from-to)1780-1787
Number of pages8
JournalCancer science
Volume103
Issue number10
DOIs
Publication statusPublished - 10-2012

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Girdin locates in centrosome and midbody and plays an important role in cell division'. Together they form a unique fingerprint.

  • Cite this

    Mao, J. Z., Jiang, P., Cui, S. P., Ren, Y. L., Zhao, J., Yin, X. H., Enomoto, A., Liu, H. J., Hou, L., Takahashi, M., & Zhang, B. (2012). Girdin locates in centrosome and midbody and plays an important role in cell division. Cancer science, 103(10), 1780-1787. https://doi.org/10.1111/j.1349-7006.2012.02378.x