Glial conditioned medium alters the expression of amyloid precursor protein in SH-SY5Y neuroblastoma cells

Yuichi Hirose, Yoshinori Imai, Kazuyuki Nakajima, Nagisa Takemoto, Shigeo Toya, Shinichi Kohsaka

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20 Citations (Scopus)


The effect of conditioned medium from astroglia or microglia cultures on the mRNA expression of amyloid precursor protein (APP) in SH-SY5Y human neuroblastoma cells was examined. When incubated with conditioned medium of glial cells, SH-SY5Y cells expressed a larger amount of APP mRNA than the control cells. Reverse transcription followed by the polymerase chain reaction showed marked induction of expression of APP isoforms containing a Kunitz-type protease inhibitor domain. Our results suggest that glial cells may contribute to the regulation of expression of APP in neurons.

Original languageEnglish
Pages (from-to)504-509
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - 31-01-1994
Externally publishedYes


All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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