Glial conditioned medium alters the expression of amyloid precursor protein in SH-SY5Y neuroblastoma cells

Yuichi Hirose; Yoshinori Imai; Kazuyuki Nakajima; Nagisa Takemoto; Shigeo Toya; Shinichi Kohsaka

doi:10.1006/bbrc.1994.1074

Glial conditioned medium alters the expression of amyloid precursor protein in SH-SY5Y neuroblastoma cells

Yuichi Hirose, Yoshinori Imai, Kazuyuki Nakajima, Nagisa Takemoto, Shigeo Toya, Shinichi Kohsaka

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

The effect of conditioned medium from astroglia or microglia cultures on the mRNA expression of amyloid precursor protein (APP) in SH-SY5Y human neuroblastoma cells was examined. When incubated with conditioned medium of glial cells, SH-SY5Y cells expressed a larger amount of APP mRNA than the control cells. Reverse transcription followed by the polymerase chain reaction showed marked induction of expression of APP isoforms containing a Kunitz-type protease inhibitor domain. Our results suggest that glial cells may contribute to the regulation of expression of APP in neurons.

Original language	English
Pages (from-to)	504-509
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	198
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.1994.1074
Publication status	Published - 31-01-1994
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1006/bbrc.1994.1074

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abstract = "The effect of conditioned medium from astroglia or microglia cultures on the mRNA expression of amyloid precursor protein (APP) in SH-SY5Y human neuroblastoma cells was examined. When incubated with conditioned medium of glial cells, SH-SY5Y cells expressed a larger amount of APP mRNA than the control cells. Reverse transcription followed by the polymerase chain reaction showed marked induction of expression of APP isoforms containing a Kunitz-type protease inhibitor domain. Our results suggest that glial cells may contribute to the regulation of expression of APP in neurons.",

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AU - Hirose, Yuichi

AU - Imai, Yoshinori

AU - Nakajima, Kazuyuki

AU - Takemoto, Nagisa

AU - Toya, Shigeo

AU - Kohsaka, Shinichi

PY - 1994/1/31

Y1 - 1994/1/31

N2 - The effect of conditioned medium from astroglia or microglia cultures on the mRNA expression of amyloid precursor protein (APP) in SH-SY5Y human neuroblastoma cells was examined. When incubated with conditioned medium of glial cells, SH-SY5Y cells expressed a larger amount of APP mRNA than the control cells. Reverse transcription followed by the polymerase chain reaction showed marked induction of expression of APP isoforms containing a Kunitz-type protease inhibitor domain. Our results suggest that glial cells may contribute to the regulation of expression of APP in neurons.

AB - The effect of conditioned medium from astroglia or microglia cultures on the mRNA expression of amyloid precursor protein (APP) in SH-SY5Y human neuroblastoma cells was examined. When incubated with conditioned medium of glial cells, SH-SY5Y cells expressed a larger amount of APP mRNA than the control cells. Reverse transcription followed by the polymerase chain reaction showed marked induction of expression of APP isoforms containing a Kunitz-type protease inhibitor domain. Our results suggest that glial cells may contribute to the regulation of expression of APP in neurons.

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Glial conditioned medium alters the expression of amyloid precursor protein in SH-SY5Y neuroblastoma cells

Abstract

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