Glial conditioned medium alters the expression of amyloid precursor protein in SH-SY5Y neuroblastoma cells

Yuichi Hirose, Yoshinori Imai, Kazuyuki Nakajima, Nagisa Takemoto, Shigeo Toya, Shinichi Kohsaka

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The effect of conditioned medium from astroglia or microglia cultures on the mRNA expression of amyloid precursor protein (APP) in SH-SY5Y human neuroblastoma cells was examined. When incubated with conditioned medium of glial cells, SH-SY5Y cells expressed a larger amount of APP mRNA than the control cells. Reverse transcription followed by the polymerase chain reaction showed marked induction of expression of APP isoforms containing a Kunitz-type protease inhibitor domain. Our results suggest that glial cells may contribute to the regulation of expression of APP in neurons.

Original languageEnglish
Pages (from-to)504-509
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume198
Issue number2
DOIs
Publication statusPublished - 31-01-1994
Externally publishedYes

Fingerprint

Amyloid beta-Protein Precursor
Conditioned Culture Medium
Neuroblastoma
Neuroglia
Messenger RNA
Polymerase chain reaction
Microglia
Transcription
Protease Inhibitors
Amyloid
Astrocytes
Reverse Transcription
Neurons
Protein Isoforms
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Hirose, Yuichi ; Imai, Yoshinori ; Nakajima, Kazuyuki ; Takemoto, Nagisa ; Toya, Shigeo ; Kohsaka, Shinichi. / Glial conditioned medium alters the expression of amyloid precursor protein in SH-SY5Y neuroblastoma cells. In: Biochemical and Biophysical Research Communications. 1994 ; Vol. 198, No. 2. pp. 504-509.
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Glial conditioned medium alters the expression of amyloid precursor protein in SH-SY5Y neuroblastoma cells. / Hirose, Yuichi; Imai, Yoshinori; Nakajima, Kazuyuki; Takemoto, Nagisa; Toya, Shigeo; Kohsaka, Shinichi.

In: Biochemical and Biophysical Research Communications, Vol. 198, No. 2, 31.01.1994, p. 504-509.

Research output: Contribution to journalArticle

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