Global expression profiles in 1-hour biopsy specimens of human kidney transplantation from donors after cardiac death

Mamoru Kusaka, Yoko Kuroyanagi, Terumi Mori, Kayuri Nagaoka, Hitomi Sasaki, Takahiro Maruyama, Kunihiro Hayakawa, Ryoichi Shiroki, Hiroki Kurahashi, Kiyotaka Hoshinaga

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Abstract

Because of the worldwide shortage of renal grafts, kidney transplantation (KTx) from donors after cardiac death (DCD) is an alternative way to obtain KTx from brain-dead donors. Although the prognosis of DCD KTx is gradually improving, the graft often undergoes delayed graft function (DGF), rendering the control of DGF essential for post-KTx patient care. In an attempt to characterize etiology of DGF, genome-wide gene expression profiling was performed using renal biopsy samples performed at 1 h after KTx from DCD and the data were compared with those of KTx from living donors (LD). A total of 526 genes were differentially expressed between them. Genes involved in acute inflammation were activated, while metabolic pathways were consistently downregulated in DCD. These findings imply the inferior performance of the DCD grafts relative to LD grafts. Several genes were identified where the expression levels were correlated well with parameters indicating short- and long-term prognosis of the DCD patients. In addition, several genes encoding secretory proteins were identified that might reflect the performance of the graft and be potential noninvasive biomarkers. These data provide a good source for candidates of biomarkers that are potentially useful for the control of DGF.

Original languageEnglish
Pages (from-to)647-656
Number of pages10
JournalCell Transplantation
Volume18
Issue number5-6
DOIs
Publication statusPublished - 2009

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All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Cell Biology
  • Transplantation

Cite this

Kusaka, M., Kuroyanagi, Y., Mori, T., Nagaoka, K., Sasaki, H., Maruyama, T., Hayakawa, K., Shiroki, R., Kurahashi, H., & Hoshinaga, K. (2009). Global expression profiles in 1-hour biopsy specimens of human kidney transplantation from donors after cardiac death. Cell Transplantation, 18(5-6), 647-656. https://doi.org/10.1177/096368970901805-621