Glomerular injury induced by antibodies against heparan sulfate proteoglycan (HSPG) derived from PYS 2 cells

Futoshi Yoshida, Seiichi Matsuo, Takeyuki Hiramatsu, Takanobu Okura, Yukio Yuzawa, Yuzo Watanabe, Nobuo Sakamoto, Seiichi Matsuo

Research output: Contribution to journalArticle

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Abstract

Kidneys of Balb/c mice intravenously injected with rabbit antibodies to heparan sulfate proteoglycan (HSPG) derived from PYS-2 cells were studied for 14 weeks. Antibodies were found to bind to the glomerular basement membrane (GBM) as early as 15 min after the injection. Binding of antibodies was observed over the whole thickness of the GBM. The lamina rara interna and foot process base appeared to be more intensely stained. This pattern did not change throughout the experiments. Mild inflammatory changes (infiltration of polymorphonuclear cells and swelling of the glomerular endothelium) were seen at the initial stage (~1 day), and mesangial expansion followed (1–4 weeks). In mice pre- and booster-immunized with normal rabbit IgG, a moderate autologous response was observed by immunofluorescence microscopy, but no significant inflammatory changes were noted. At the late stage (6 weeks ~), irregular GBM thickening was observed. The thickening was due mainly to expansion of the lamina rara externa. These findings suggest that the anti-HSPG antibodies caused mild glomerulonephritis at the initial stage, and later caused thickening of the GBM possibly by disturbing the assembly, production and degradation of GBM components.

Original languageEnglish
Pages (from-to)135-143
Number of pages9
JournalThe Japanese Journal of Nephrology
Volume31
Issue number2
DOIs
Publication statusPublished - 01-01-1989

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Heparan Sulfate Proteoglycans
Glomerular Basement Membrane
Antibodies
Wounds and Injuries
Rabbits
Glomerulonephritis
Fluorescence Microscopy
Endothelium
Foot
Immunoglobulin G
Kidney
Injections

All Science Journal Classification (ASJC) codes

  • Nephrology

Cite this

Yoshida, Futoshi ; Matsuo, Seiichi ; Hiramatsu, Takeyuki ; Okura, Takanobu ; Yuzawa, Yukio ; Watanabe, Yuzo ; Sakamoto, Nobuo ; Matsuo, Seiichi. / Glomerular injury induced by antibodies against heparan sulfate proteoglycan (HSPG) derived from PYS 2 cells. In: The Japanese Journal of Nephrology. 1989 ; Vol. 31, No. 2. pp. 135-143.
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abstract = "Kidneys of Balb/c mice intravenously injected with rabbit antibodies to heparan sulfate proteoglycan (HSPG) derived from PYS-2 cells were studied for 14 weeks. Antibodies were found to bind to the glomerular basement membrane (GBM) as early as 15 min after the injection. Binding of antibodies was observed over the whole thickness of the GBM. The lamina rara interna and foot process base appeared to be more intensely stained. This pattern did not change throughout the experiments. Mild inflammatory changes (infiltration of polymorphonuclear cells and swelling of the glomerular endothelium) were seen at the initial stage (~1 day), and mesangial expansion followed (1–4 weeks). In mice pre- and booster-immunized with normal rabbit IgG, a moderate autologous response was observed by immunofluorescence microscopy, but no significant inflammatory changes were noted. At the late stage (6 weeks ~), irregular GBM thickening was observed. The thickening was due mainly to expansion of the lamina rara externa. These findings suggest that the anti-HSPG antibodies caused mild glomerulonephritis at the initial stage, and later caused thickening of the GBM possibly by disturbing the assembly, production and degradation of GBM components.",
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Glomerular injury induced by antibodies against heparan sulfate proteoglycan (HSPG) derived from PYS 2 cells. / Yoshida, Futoshi; Matsuo, Seiichi; Hiramatsu, Takeyuki; Okura, Takanobu; Yuzawa, Yukio; Watanabe, Yuzo; Sakamoto, Nobuo; Matsuo, Seiichi.

In: The Japanese Journal of Nephrology, Vol. 31, No. 2, 01.01.1989, p. 135-143.

Research output: Contribution to journalArticle

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AU - Matsuo, Seiichi

AU - Hiramatsu, Takeyuki

AU - Okura, Takanobu

AU - Yuzawa, Yukio

AU - Watanabe, Yuzo

AU - Sakamoto, Nobuo

AU - Matsuo, Seiichi

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N2 - Kidneys of Balb/c mice intravenously injected with rabbit antibodies to heparan sulfate proteoglycan (HSPG) derived from PYS-2 cells were studied for 14 weeks. Antibodies were found to bind to the glomerular basement membrane (GBM) as early as 15 min after the injection. Binding of antibodies was observed over the whole thickness of the GBM. The lamina rara interna and foot process base appeared to be more intensely stained. This pattern did not change throughout the experiments. Mild inflammatory changes (infiltration of polymorphonuclear cells and swelling of the glomerular endothelium) were seen at the initial stage (~1 day), and mesangial expansion followed (1–4 weeks). In mice pre- and booster-immunized with normal rabbit IgG, a moderate autologous response was observed by immunofluorescence microscopy, but no significant inflammatory changes were noted. At the late stage (6 weeks ~), irregular GBM thickening was observed. The thickening was due mainly to expansion of the lamina rara externa. These findings suggest that the anti-HSPG antibodies caused mild glomerulonephritis at the initial stage, and later caused thickening of the GBM possibly by disturbing the assembly, production and degradation of GBM components.

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